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Method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase

A technology of Bacteroides pilosa and ginsenoside, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., and can solve the problems of complicated liquid fermentation processes, etc.

Pending Publication Date: 2021-02-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN105925655A discloses a method for preparing rare ginsenosides by liquid fermentation of Pinus fir ganoderma lucidum, and the process of liquid fermentation is relatively complicated

Method used

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  • Method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase
  • Method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase
  • Method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The hard leathery bacteria is inoculated in the solid-state fermentation medium according to the inoculation amount of 20% (100g then 20mL), and the formula of the solid-state fermentation medium is: 20g of corn bran is the fermentation substrate, and the feeding amount of the ginseng extract is 0.2g / g 玉米皮 , The initial moisture content is 60%. The solid-state fermentation culture was carried out at 20°C for 10 days.

[0050] After the fermentation, the fermentation product was leached with acetic acid buffer (pH=6.0, 50mM) at a ratio of 1g:5mL for 12 hours at 4°C, the extract was centrifuged (10000rpm, 20min) to filter mycelia and impurities, and the supernatant was collected As a crude enzyme solution, it is used to measure the enzyme activity of β-glucosidase. When determining the content of ginsenosides, the fermentation product was extracted with equal volume of n-butanol for 3 times, each time for 12 hours, the n-butanol extract was evaporated to dryness on a vac...

Embodiment 2

[0052] The hard leathery bacteria was inoculated into the solid-state fermentation medium according to the inoculum size of 20% (100g and 20mL), and the cellulase (enzyme activity was 50U / g) derived from Aspergillus niger was inoculated according to the solid-state fermentation medium 2.5% (w / The proportion of w) is added in the solid-state fermentation medium, and the formula of the solid-state fermentation medium is: 20g corn bran is the fermentation substrate, and the feeding amount of ginseng extract is 0.2g / g 玉米皮 , The initial moisture content is 60%. Fermentation was carried out at 20°C for 10 days.

[0053] After the fermentation, the fermentation product was leached with acetic acid buffer (pH=6.0, 50mM) at a ratio of 1g:5mL for 12 hours at 4°C, the extract was centrifuged (10000rpm, 20min) to filter mycelia and impurities, and the supernatant was collected As a crude enzyme solution, it is used to measure the enzyme activity of β-glucosidase. When determining the c...

Embodiment 3

[0055] The hard leathery bacteria was inoculated into the solid-state fermentation medium at an inoculum size of 20%, and the cellulase (15000U / g) derived from Trichoderma viride was added to the solid-state fermentation medium according to the ratio of 2.5% (w / w) of the solid-state fermentation medium. In the fermentation medium, the formula of the solid-state fermentation medium is: 20g of corn bran as the fermentation substrate, and the dosage of ginseng extract is 0.2g / g 玉米皮 , The initial moisture content is 60%. Fermentation was carried out at 20°C for 10 days.

[0056] After the fermentation, the fermented product was leached with acetic acid buffer solution (pH=6.0, 50mM) at a ratio of 1:5 for 12 hours at 4°C, and the extract was centrifuged (10000rpm, 20min) to filter mycelium and impurities, and the supernatant was collected As a crude enzyme solution, it is used to measure the enzyme activity of β-glucosidase. When determining the content of ginsenosides, the ferme...

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Abstract

The invention discloses a method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase, and belongs to the technical field of biological preparation of theginsenoside. According to the method, the stereum hirsutum is taken as a fermentation strain, a seed solution is inoculated into a culture medium which takes agricultural waste as a fermentation substrate and contains ginseng extract and cellulase, solid-state fermentation culture is performed for 10 days, and a product rich in ginsenoside Rd, F2, CK, PPT and other rare saponins is obtained afterfermentation is finished, and meanwhile, the fermentation product also contains a plurality of bioactive substances such as stereum hirsutum polysaccharides, sterols, esters and sesquiterpenes. The method is simple in process, low in fermentation cost and high in conversion efficiency, provides a reference for circular biological economic production of the bioactive substances containing various rare ginsenosides, and provides a method for developing a new technology for preparing various high-value health products by utilizing fermentation of the stereum hirsutum.

Description

technical field [0001] The present invention relates to a method for preparing rare ginsenosides by solid-state fermentation of C. villosa and cellulase, especially a technique for preparing rare ginsenosides by solid-state fermentation of ginseng extracts by mixing C. villosa and cellulase, which belongs to the category of ginsenosides. Biotechnology field. Background technique [0002] Ginseng is a traditional Chinese traditional medicine, which can also be used for dietotherapy. The main active ingredient of ginseng is ginsenoside, especially the rare ginsenoside has higher pharmacological activity because of its lower molecular weight, better cell membrane permeability and easier absorption by the gastrointestinal tract. More than 150 kinds of ginsenosides and their derivatives have been identified from different parts of American ginseng, ginseng products and other American ginseng. All ginsenosides have the same basic structure, that is, 4 rings consisting of a damma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 余晓斌杨文华顾秋亚周剑丽
Owner JIANGNAN UNIV
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