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Preparation method of adenosylmethionine butanedisulfonate with health-care functions

A technology of adenosylmethionine butanedisulfonate and adenosylmethionine butanedisulfonate is applied in the field of preparation of adenosylmethionine butanedisulfonate, and can solve the problems of no application prospect, low yield, research on finished products of adenosine methionine butanedisulfonate and the like , to achieve the effect of improving catalytic activity, high reaction efficiency and high yield

Active Publication Date: 2021-02-19
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to its disclosed content, it can be seen that it has not studied the yield of adenosylmethionine succinate. If the purity of the product in actual production is high, but the yield is low, there is still no application prospect

Method used

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  • Preparation method of adenosylmethionine butanedisulfonate with health-care functions
  • Preparation method of adenosylmethionine butanedisulfonate with health-care functions
  • Preparation method of adenosylmethionine butanedisulfonate with health-care functions

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Experimental program
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Effect test

Embodiment 1-3

[0031] Embodiment 1-3 A method for preparing adenosylmethionine succinate for health care function, comprising the following steps:

[0032] (1) Inserting the adenosylmethionine-producing bacteria into the activation medium at a culture temperature of 24° C. and culturing for 5 days to obtain an activated strain;

[0033] (2) Inoculate the activated bacterial strain obtained in step (1) in the first-level seed medium, cultivate the temperature for 12 hours at 24° C., and obtain the first-level seed; then inoculate the first-level seed into the next-level seed medium , cultured at 24°C for 12 hours to obtain secondary seeds;

[0034] (3) Insert the secondary seeds obtained in step (2) into the fermentation medium (fermentation liquid volume 50 L), the inoculum size is 25%, and the cultivation temperature is 24° C. After 15 hours of fermentation, add adenosine triphosphate and L - Methionine, co-fermented for 40 hours to obtain adenosylmethionine fermentation liquid;

[0035] ...

Embodiment 4-6

[0047] Embodiment 4-6 A kind of preparation method of succinate adenosylmethionine for health care function, comprises the following steps:

[0048] (1) Inoculating the adenosylmethionine-producing bacteria into the activation medium at a culture temperature of 32°C for 3 days to obtain the activated strain;

[0049] (2) Inoculate the activated bacterial strain obtained in step (1) in the first-level seed culture medium, cultivate the temperature at 32°C for 25 hours, and obtain the first-level seeds; then inoculate the first-level seeds into the next-level seed culture medium , cultured at 32°C for 25 hours to obtain secondary seeds;

[0050] (3) Insert the secondary seeds obtained in step (2) into the fermentation medium (50 L of fermentation broth volume), the inoculum size is 25%, and the culture temperature is 32° C. After 20 h of fermentation, add adenosine triphosphate and L - Methionine, co-fermented for 40 hours to obtain adenosylmethionine fermentation broth;

[00...

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Abstract

The invention provides a preparation method of adenosylmethionine butanedisulfonate with health-care functions, and belongs to the technical field of pharmacy. According to the preparation method of the adenosylmethionine butanedisulfonate with the health-care functions, proline, fumaric acid and Na(2)HPO(4)-12H(2)O are added into afermentation medium at a content ratio of 2-4 to 1-2 to 1-2 duringthe implementation process so as to have expression level of s-adenosylmethionine synthetase improved with catalytic activity of the s-adenosylmethionine synthetase in a living body increased; and thus, improvement of L-methionine conversion rate can be guaranteed with addition of adenosine triphosphate at a relatively low content in a fermentation system. Reaction efficiency of the obtained adenosylmethionine with butanedisulfonic acid is high, so that the finally prepared adenosylmethionine butanedisulfonate product has obviously improved purity.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a preparation method of adenosylmethionine succinate for health care function. Background technique [0002] Adenosylmethionine succinate is a physiologically active molecule ubiquitously present in human tissues and body fluids. As a methyl donor (transmethylation) and a precursor of physiological sulfhydryl compounds (such as cysteine, taurine, glutathione and coenzyme A, etc.) biochemical reactions. It is clinically used to treat depression, arthritis, liver dysfunction and other diseases. It is also suitable for intrahepatic cholestasis caused by pre-cirrhosis and cirrhosis, and intrahepatic cholestasis during pregnancy. Its pharmacological action is as follows: adenosylmethionine is a physiologically active molecule present in all tissues and body fluids of the human body. As a methyl donor (transmethylation) and a precursor of physiological sulfur compounds ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12N1/16C07H19/167C07H1/00C07C303/32C07C309/05C12R1/865
CPCC12P19/40C12N1/16C07H19/167C07H1/00C07C303/32C07C309/05
Inventor 周彦乐杨宝雄郑国强王国强
Owner 内蒙古拜克生物有限公司
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