Fixed bed bioreactor

A technology of bioreactor and fixed bed, which is applied in the direction of specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, method of supporting/immobilizing microorganisms, etc., which can solve the problem of insufficient mixing and insufficient absorption of cells Nutrients, slow cell growth and other issues, to achieve the effect of full absorption, increase the concentration of cell culture, and avoid inhibition

Inactive Publication Date: 2021-02-19
SHINVA MEDICAL INSTR CO LTD
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this culture method, the medium newly entering the fixed bed cannot be fully mixed with the original medium in the fixed bed, and the accumulation of the carrier causes the...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fixed bed bioreactor
  • Fixed bed bioreactor
  • Fixed bed bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] Figure 1~8 It is the best embodiment of the present invention, below in conjunction with attached Figure 1~8 The present invention will be further described.

[0029] Such as figure 1 As shown, a fixed bed bioreactor comprises a tank body 6 with an open upper end, a tank cover 5 is arranged at the upper port of the tank body 6, and a plurality of protruding tank cover 5 upper surfaces are provided on the tank cover 5 interface 12 (see Figure 4 ), refer to image 3 A dissolved oxygen detector 1, an air supply pipe 2, a pH detection pipe 3, an acid-base defoaming adding pipe 4, a sampling pipe 7 and a feeding pipe 11 are installed on the tank cover 5 through the interface 12, the dissolved oxygen detector 1, the replenishing pipe The top of the air pipe 2, the pH detection tube 3, the acid-base defoaming adding tube 4, the sampling tube 7 and the feeding tube 11 are located above the tank cover 5, and the lower end thereof passes through the tank cover 5 along the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a fixed bed bioreactor, and belongs to the technical field of cell culture. The bioreactor comprises a tank body (6); a tank cover (5) is arranged at an upper port of the tank body (6); and a micro-carrier (9) is fixed in the tank body (6). The bioreactor is characterized in that a placing tower (8) is arranged in the tank body (6); the micro-carrier (9) is placed on an outer ring of the placing tower (8); circulating channels of a culture medium are formed between the placing tower (8) and the inner wall of the tank body (6) as well as inside the placing tower (8); anda circulation mechanism (10) for circulating the culture medium inside the circulating channels is arranged at the bottom of the placing tower (8). In the fixed bed bioreactor, the circulating mechanism is arranged, and the culture medium is circulated in the tank body by virtue of the circulating mechanism, so that when the culture medium flows upwards from the bottom of the micro-carrier, the carrier is loosened by virtue of the flowing impact force of the culture medium, and cells can absorb nutrients more favorably.

Description

technical field [0001] A fixed-bed bioreactor belongs to the technical field of cell culture. Background technique [0002] Fixed bed bioreactor is a new type of cell culture device, which can realize up to 10 8 The high-concentration cell culture level per mL is about 10 times that of other conventional methods (suspension culture). The existing fixed bed reactor includes a carrier fixing device and a liquid storage tank. The medium is pumped to the top of the fixed bed through a peristaltic pump, and the medium permeates through the fixed bed and returns to the liquid storage tank from the bottom of the fixed bed, and the cycle is repeated in turn. . In this culture method, the medium newly entering the fixed bed cannot be fully mixed with the original medium in the fixed bed, and the accumulation of the carrier causes the cells inside the carrier to fail to fully absorb nutrients, resulting in slow cell growth and low cell density after the end of the culture. , affect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12M1/02C12M1/04C12M1/34C12M1/21C12M1/00
CPCC12M25/18C12M27/02C12M29/18C12M29/00C12M33/04C12M33/00C12M41/26C12M41/34C12M41/02C12M23/48
Inventor 丁小锋李晓明李之良黄利军
Owner SHINVA MEDICAL INSTR CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap