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Method for extracting genistein and glucoside thereof

An extraction method and genistein technology, applied in the field of natural products, can solve problems such as poor reproducibility of results

Active Publication Date: 2021-02-12
重庆市六九畜牧科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction yield and purity of genistin obtained by this method are sign

Method used

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  • Method for extracting genistein and glucoside thereof
  • Method for extracting genistein and glucoside thereof

Examples

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Embodiment 1

[0041] Pulverize the pre-dried ginseng root, pass through a 280-mesh sieve, and take 10 g of the sifted powder as the raw material for extraction. The extraction raw material is placed in a negative pressure cavitation extraction tank, which uses 70v% methanol aqueous solution as the extraction solvent; the solid-liquid ratio of the extraction raw material to the extraction solvent is 1:45 (g / g). The negative pressure cavitation extraction tank and its circulation pipeline are placed in the water bath of the ultrasonic generator. The working power of ultrasonic operation is 350W; the working frequency is 33kHz. The pressure of negative pressure cavitation operation is -0.08MPa. The extraction temperature is 40°C; the extraction time is 40min. Then, the extract was centrifuged and the supernatant was taken. Methanol in the supernatant was removed by rotary evaporation; then the remaining liquid was vacuum-dried at 50°C until the mass was constant to obtain an extract powder ...

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Abstract

The invention discloses a method for extracting genistein and glucoside thereof, which comprises the following steps: by using flemingia macrophylla root as an extraction raw material and a polar solvent as an extraction solvent, extracting the extraction raw material by using ultrasonic negative-pressure cavitation synergistic operation. By using the specific ultrasonic negative-pressure cavitation synergistic operation, the extraction rate and purity of the genistein and glucoside thereof are higher, and the result reproducibility of the method is better.

Description

technical field [0001] The invention belongs to the technical field of natural products, and in particular relates to a method for extracting genistein and its glycosides. Background technique [0002] The chemical name of genistein is 4’,5,7-trihydroxyisoflavone, genistein, genistein or genistein, and it is the most biologically active of isoflavones. The molecular structure of genistein has one isoflavone ring and one phenolic ring, and there are three hydroxyl groups at the 5,7-position of the isoflavone ring and the 4'-position of the phenolic ring. [0003] Under natural conditions, genistein exists in plants in the form of aglycones and glycosides. After entering the human and animal bodies, genistein glycosides are catalyzed by glycosidases in the pancreas and small intestine to produce related aglycones, which are further converted into p-ethylphenol under the action of intestinal juice bacteria. Finally, p-ethylphenol is absorbed through the intestinal epithelium ...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40C07H17/07C07H1/08B01D11/02
CPCC07D311/36C07D311/40C07H17/07C07H1/08B01D11/0265B01D11/0207B01D11/0292B01D11/0288
Inventor 罗云蒲强
Owner 重庆市六九畜牧科技股份有限公司
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