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Method for quickly detecting tert-butylhydroquinone in food

A tertiary butyl hydroquinone, food technology, applied in the field of chemical analysis and detection, can solve the problems of catalytic oxidation, which is rarely reported, and achieve the effect of fast reaction, high sensitivity and good reproducibility

Active Publication Date: 2021-02-09
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on carbon dots as nanozymes, and the use of carbon dots to prepare gold nanoparticles has also been reported, but there are few reports on the composite nanozymes formed by the combination of the two and the catalytic oxidation of TBHQ as a substrate.

Method used

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  • Method for quickly detecting tert-butylhydroquinone in food
  • Method for quickly detecting tert-butylhydroquinone in food
  • Method for quickly detecting tert-butylhydroquinone in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the mensuration of TBHQ in the soybean oil sample

[0023] 1. Preparation of Cu-I / CDs: 0.1g CuCl 2 , 0.4g of 3-iodo-L-tyrosine was added to 40mL of ultrapure water, mixed and dissolved, then 100μL of ethylenediamine was added, ultrasonicated for 10min, and transferred to a polytetrafluoroethylene reactor, heated at 180°C for 8h, naturally After cooling, first filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3000 Da for 24 hours to obtain water-soluble Cu-I / CDs;

[0024] 2. Gold nanoparticles prepared by Cu-I / CDs reduction: add 2 mL of polypropyleneimine with a concentration of 0.25 g / mL to 20 mL of ultrapure water, add 80 μL of chloroauric acid with a mass concentration of 1% under stirring, and then add 200 μL Cu-I / CDs, after stirring for 30min, the solution turns reddish brown, namely the gold nanometer prepared by reduction of Cu-I / CDs.

[0025] 3. Pre...

Embodiment 2

[0035] Example 2: Determination of TBHQ in sunflower oil samples

[0036] 1. Preparation of Cu-I / CDs: 1 g CuCl 2 Dissolve 3g of 3-iodo-L-tyrosine in 500mL of purified water, mix and dissolve, add 900μL of ethylenediamine, ultrasonicate for 15min, transfer to a polytetrafluoroethylene reactor, heat at 180°C for 10h, cool naturally, and Filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3000D for 24 hours to obtain water-soluble Cu-I / CDs;

[0037] 2. Preparation of gold nanoparticles obtained by reduction of Cu-I / CDs: 2 mL of 0.25 g / mL polypropyleneimine, 18 mL of ultrapure water, 90 μL of 1% chloroauric acid was added under stirring, and then 190 μL of Cu-I / CDs, after stirring for 35min, the solution turned reddish brown, and the obtained Cu-I / CDs were reduced to obtain gold nanometers;

[0038] 3, tert-butylhydroquinone working curve is made with embodiment 1;

[0039] 4. Det...

Embodiment 3

[0042] Example 3: Determination of TBHQ in Cookies

[0043] 1, the preparation of Cu-I / CDs is the same as embodiment 1;

[0044] 2, the preparation of the gold nanometer that Cu-I / CDs reduction makes is the same as embodiment 1;

[0045] 3, tert-butylhydroquinone working curve is made with embodiment 1;

[0046] 4. Determination of TBHQ in cookies

[0047] (1) Sample treatment: Weigh 5.00g of evenly powdered biscuit sample into a 100mL Erlenmeyer flask, add 8mL of absolute ethanol, vortex mix for 1min, then ultrasonically extract for 15min, let stand for stratification, and draw the supernatant into a 50mL centrifuge tube , the residue was extracted twice with 8 mL of absolute ethanol each time; the combined supernatant was added to a 50 mL centrifuge tube with 1 g of neutral alumina, vortexed for 1 min, and centrifuged at 3000 r / min for 8 min, and the supernatant was pipetted into a 25 mL capacity In the bottle, dilute to volume with absolute ethanol, shake well, and obtai...

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Abstract

The invention discloses a method for quickly detecting tert-butylhydroquinone in food, which comprises the following steps: reducing chloroauric acid by using copper iodine doped carbon dots as a reducing agent and polypropyleneimine as a protective agent to obtain gold nanoparticles, forming a mixed nano-enzyme system by using excessive Cu-I / CDs and AuNPs, and establishing a novel high-sensitivity and high-selectivity TBHQ detection method by using tert-butylhydroquinone as a substrate and oxidized TBHQ as a red oxidized quinones substance based on the linear relationship between the TBHQ concentration and the red oxidized TBHQ, wherein the detection limit is 0.5 mg / kg; the method is applied to the detection and analysis of TBHQ in food, and the result conforms to the determination methodof nine antioxidants in food of the national food safety standard GB5009.32-2016; the method can only selectively oxidize TBHQ, other antioxidants do not have the reaction, and the method has the characteristics of high sensitivity, strong specificity, simplicity and rapidness in operation and the like.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for rapidly detecting tert-butylhydroquinone in food. Background technique [0002] Tert-butyl hydroquinone (Tert-butyl hydroquinone, TBHQ) is a relatively common antioxidant in food. Oxidation performance is stronger; in addition, TBHQ is relatively stable to heat, and it does not have any peculiar smell when added to oil samples. However, TBHQ has certain toxicity to the human body. Long-term storage or repeated cooking will decompose TBHQ, and its metabolite tert-butyl-p-benzoquinone is highly toxic. GB2760-2014 clearly stipulates that the maximum amount of TBHQ added to edible vegetable oil is 200mg / kg. Common detection methods for TBHQ include colorimetry, liquid chromatography, gas chromatography, gas chromatography, etc. Colorimetry is often used because it is simple, fast, and does not require large-scale equipment. The interference is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/31G01N1/28
Inventor 杨德志李秋兰陈子昭杨亚玲
Owner KUNMING UNIV OF SCI & TECH
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