Method for quantitatively detecting content of free DNA by isotope dilution mass spectrometry
A technology of isotope dilution and content, applied in the biological field, can solve the problems of improving preparation, low analysis speed and analysis throughput, etc.
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[0085] A method of isotope dilution ultra-high performance liquid chromatography-mass spectrometry for the determination of free DNA single strands in serum in this embodiment, the experimental flow chart is as follows figure 1 shown, including the following steps:
[0086] (1) Preparation of free DNA single-strand nucleic acid single-strand solution, peptide nucleic acid probe solution and isotope-labeled polypeptide internal standard solution: the amount of free DNA single-strand synthesis is 2OD value, and 380 μL DEPC water is accurately added to it to prepare a concentration of 20 μmol / Transfer L of free ssDNA solution to a 2.0 mL centrifuge tube. Take 100 μL, add 900 μL of DEPC water to dilute to a concentration of 2 μmol / L as a stock solution and dispense into 2.0 mL centrifuge tubes, each tube is dispensed with 100 μL, and place the distributed free DNA single-strand solution in- Store in refrigerator at 70°C. Before use, dilute the stock solution with DEPC water to ...
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