Rapid detection kit for novel coronavirus antigen, preparation method and application of rapid detection kit

Abstract

The invention discloses a rapid detection kit for a novel coronavirus antigen. The rapid detection kit comprises a kit body and a reagent strip placed in the kit body. The reagent strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption padare sequentially overlapped end to end along the length direction of the bottom plate. The combination pad is coated with an anti-nucleocapsid protein monoclonal antibody and an anti-spinous glycoprotein monoclonal antibody which are labeled by colloidal gold; and the nitrocellulose membrane is coated with a nucleocapsid protein detection line N line formed by an anti-nucleocapsid protein monoclonal antibody, a spinous glycoprotein detection line S line formed by an anti-spinous glycoprotein monoclonal antibody, and a quality control line C line formed by a goat anti-mouse IgG antibody. The invention also discloses a preparation method and an application of the kit. The rapid detection kit prepared in the invention can be used for directly detecting the novel coronavirus, is simple and convenient to operate and short in detection time, and is suitable for large-scale population screening and on-site screening.

Description

technical field [0001] The invention belongs to the technical field of biological immune detection and analysis, and in particular relates to a rapid detection kit for a novel coronavirus antigen and a preparation method and application thereof. Background technique [0002] The novel coronavirus (2019-nCoV) that emerged at the end of 2019 has attracted widespread attention from all walks of life. The virus can be transmitted through respiratory droplets and contact. [0003] At present, there are two main detection methods for the new coronavirus: nucleic acid detection and antibody detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity, and is the "gold standard" for the diagnosis of new coronary pneumonia. However, nucleic acid detection requires relatively high detection equipment. High, it can only be carried out in a laboratory with relatively perfect conditions, and the average detection time needs to be more tha...

AI Technical Summary

Problems solved by technology

[0003] At present, there are two main detection methods for the new coronavirus: nucleic acid detection and antibody detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity, and is the "gold standard" for the diagnosis of new coronary pneumonia, but nucleic acid detection requires relatively high detection equipment. High, it can only be carried out in a laboratory with relatively perfect conditions, and the average detection time takes more than 4 hours, the operation is complicated, and it depends on real-time quantitative PCR instrument and high-level technical personnel; the advantage of antibody detection is that it is convenient and fast to use, and the detection The time is short, which can effectively break through the limitations of existing detection technology on personnel and places, and shorten the detection time

However, there is a window period for antibody production, which leads to false negative results in antibody testing, and IgG antibodies exist for a long time, which cannot be used for convalescence examinations, nor can it effectively distinguish between previously infected people and existing infected people

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