Halbach array type anti-magnetic levitation cell density detection and separation device and method

Abstract

The invention discloses a Halbach array type anti-magnetic levitation cell density detection and separation device and method. The device comprises a fixed base, a separation assembly, a magnet assembly and an observation instrument fixedly arranged through the magnet assembly, wherein the separation assembly and the magnet assembly are arranged on the fixed base and are used for separating and extracting cells; the magnet assembly with a clamping groove type structure is movably arranged on the fixed base so as to adapt to shooting positions of different observation instruments. According tothe invention, the intrinsic difference between normal cells and diseased cells is characterized based on the difference in cell intrinsic density, and high-precision, high-flux, simple operation andlow-cost non-labeled detection and non-contact separation control of cells are realized through regulation and control of a magnetic field and the flow rate of a solution; and rapid separation and nondestructive extraction of cells are realized by controlling the flow rate of the solution and the type and concentration of the paramagnetic solution.

Description

technical field [0001] The invention relates to a technology in the field of bioengineering, in particular to a Halbach array anti-magnetic levitation cell density detection and separation device and method. Background technique [0002] Cell separation methods mainly include centrifugation, flow cytometry and cell electrophoresis. Among them, although the centrifugation method is easy to use and low in cost, the accuracy of cell separation is low. The flow cytometry method has a powerful sorting function, accurate and fast detection, but the cost of the instrument is high and the operation is difficult. But it has high requirements on the dielectric and the speed analysis is difficult. [0003] Most of the cell separation methods currently in use have their own limitations, and cannot meet the different requirements for the performance of cell separation devices in different environments such as hospitals and laboratories. A simple and low-cost cell density detection and ...

AI Technical Summary

Problems solved by technology

Among them, although the centrifugation method is easy to use and low in cost, the accuracy of cell separation is low. The flow cytometry method has a powerful sorting function, accurate and fast detection, but the cost of the instrument is high and the operation is difficult. However, it has high requirements on the dielectric and difficult speed analysis

[0003] Most of the cell separation methods currently in use have their own limitations, and cannot meet the different requirements for the performance of cell separation devices in different environments such as hospitals and laboratories. Simple and low-cost cell density detection and separation device is of great significance

[0004] In recent years, some scholars have proposed operations such as density detection, quality monitoring, and separation of macroscopic objects based on the principle of magnetic levitation, which has verified the effectiveness and robustness of magnetic levitation devices in the field of density detection and separation, but for microscopic biological systems such as cells, there are still Stable suspension time is long, cell viability is easily damaged, etc.

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