Composite microbial agent for inhibiting pathogenic bacteria as well as preparation method and application of composite microbial agent
A technology of compound microbial inoculants and pathogenic bacteria, applied in the field of biological bacteria, can solve problems such as poor effect and singleness, and achieve the effects of improving killing effect, promoting decomposition, and good preventive and therapeutic effects.
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Embodiment 1
[0029] (1) 20 parts by weight of Bacillus subtilis, 15 parts of Streptomyces jingyang, 15 parts of Pseudomonas fluorescens and 2 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.
[0030] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.
Embodiment 2
[0032] (1) 25 parts by weight of Bacillus subtilis, 20 parts of Streptomyces jingyang, 20 parts of Pseudomonas fluorescens and 4 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.
[0033] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.
Embodiment 3
[0035] (1) 30 parts by weight of Bacillus subtilis, 25 parts of Streptomyces jingyang, 25 parts of Pseudomonas fluorescens and 6 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.
[0036] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.
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