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Kit for auxiliary diagnosis of colorectal cancer and adenoma and application method thereof

A colorectal cancer and auxiliary diagnosis technology, applied in the fields of biotechnology and medicine, can solve the problems of greater differentiation of colorectal adenoma, insufficient colonoscopy examination rate, and low fecal occult blood screening rate, so as to improve the level of medical services, The effect of increasing acceptance

Pending Publication Date: 2021-02-02
GENETALKS BIO TECH CHANGSHA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Statistics show that the screening rate of fecal occult blood in my country is less than 5%, and the rate of colonoscopy is less than 3%.
[0005] Based on the advantages and disadvantages of the above-mentioned existing laboratory diagnostic methods, it can be seen that there are obvious differences in the detection rate (sensitivity) and specificity of different colorectal cancer detection methods and the combined detection of different genes in the same detection method for colorectal cancer. For colorectal adenoma, the detection effect is even more different

Method used

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  • Kit for auxiliary diagnosis of colorectal cancer and adenoma and application method thereof
  • Kit for auxiliary diagnosis of colorectal cancer and adenoma and application method thereof
  • Kit for auxiliary diagnosis of colorectal cancer and adenoma and application method thereof

Examples

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Effect test

Embodiment 1

[0056] A kit for auxiliary diagnosis of colorectal cancer and adenoma of the present invention, comprising primer composition, probe composition, closed sequence composition, Hot start Taq enzyme, 10×buffer, dNTPs, NH 4 Cl, DMSO and deionized water. Wherein, the components of the primer composition are listed in Table 1, the components of the probe composition are listed in Table 2, and the components of the blocking sequence composition are listed in Table 3.

[0057] The primer composition of table 1 embodiment 1

[0058] BS-N4-F 5'-GTTTCGCGTCGCGGTTTTCGTTC-3' (SEQ ID NO.1) BS-N4-R 5'-CGTAACTTCCGCCTTCTACGCG-3' (SEQ ID NO.2) BS-SDC2-F 5'-AGAAATTAATAAGTGAGAGGGCGTCGC-3' (SEQ ID NO.3) BS-SDC2-R 5'-AACGCTCGCTTCCTCCTCCTACG-3' (SEQ ID NO.4) BS-ACT-F 5'-GAAAGGGTGTAGTTTTGGGAGGTTAG-3' (SEQ ID NO.5) BS-ACT-R 5'-CCCAAAACCAACCACAAAAAAAAT-3' (SEQ ID NO.6)

[0059] In Table 1, the primer pair BS-N4-F and BS-N4-R are used to amplify the NDR...

Embodiment 2

[0104] In addition, a total of 455 colorectal cancer and adenoma pathologically positive and pathologically negative samples with effective detection results were collected, including 57 colorectal cancer positive samples, 398 non-colorectal cancer samples, 22 polyp samples, and 298 cases of inflammation and internal hemorrhoids. Example, 58 examples of normal samples) the method established in Example 1 is verified, and compared with the actual value, the results show that the sensitivity is 0.8947, the specificity is 0.9196, the adenoma sensitivity is 0.4833, and Kappa is 0.9165. Figure 4 It is a schematic diagram of the curve of the sample detection internal standard ACTB, Figure 5 Schematic diagram of the positive sample detection curve.

[0105] Table 6 sample matrix table

[0106]

[0107]Based on the above analysis, the joint detection of SDC2 and NDRG4 genes can detect colorectal cancer and colorectal adenoma very well at the same time, and it is obviously better...

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Abstract

The invention discloses a kit for auxiliary diagnosis of colorectal cancer and adenoma and an application method thereof. The kit comprises a primer composition, a probe composition and a closed sequence composition. The primer composition is composed of BS-N4-F, BS-N4-R, BS-SDC2-F, BS-SDC2-R, BS-ACT-F and BS-ACT-R. The probe composition is composed of BS-N4-P, BS-SDC2-P and BS-ACT-P. The closed sequence composition is composed of BS-N4-C and BS-SDC2-C. The application method comprises the steps of sampling, extracting, converting, detecting and result analysis. By combining NDRG4 gene with SDC2 gene for detection and using ACTB gene as an internal reference, high detection rate and high specificity of colorectal cancer and adenoma are realized, and the result analysis is efficient and accurate in the using process. The kit of the invention has good commercial value and practical significance.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and relates to a kit and a use method thereof, in particular to a kit for auxiliary diagnosis of colorectal cancer and a use method thereof. Background technique [0002] Colorectal cancer, also known as colorectal cancer (CRC), is one of the most common clinical malignant tumors, and usually develops slowly. It has become one of the malignant tumors with the fastest-rising incidence rate in my country, seriously threatening the lives and health of Chinese people. According to the latest "China Cancer Registration Annual Report" issued by the National Cancer Registry Center, the incidence and death of colorectal cancer and other digestive tract cancers are increasing year by year nationwide, the number of new cases is about 400,000 per year, and the number of deaths is about 195,000 people / year. Especially in high-incidence areas such as the Yangtze River Delta, Pearl River Delta, Hong...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2600/166C12Q2531/113C12Q2563/107C12Q2545/101C12Q2523/125
Inventor 戴应宋卓高堂杰王益民何庆周巧
Owner GENETALKS BIO TECH CHANGSHA CO LTD
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