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Hair follicle microarray co-culture system and application in medicine for treating pathological alopecia

A pathological alopecia and co-cultivation technology, applied in the field of genetic engineering technology and biopharmaceuticals, can solve the problems of long time period and complicated operation of separation and culture

Active Publication Date: 2021-02-02
HUAKE TONGJI STEM CELL GENETIC ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, although hair follicle stem cells have clear cell surface marker molecules, the operation of their isolation and culture is more complicated and the time period is longer

Method used

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  • Hair follicle microarray co-culture system and application in medicine for treating pathological alopecia
  • Hair follicle microarray co-culture system and application in medicine for treating pathological alopecia
  • Hair follicle microarray co-culture system and application in medicine for treating pathological alopecia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Hair follicle regeneration technology patient condition records

[0058] The patient, male, 31 years old, was admitted to the hospital due to "hair loss for many years". The hair loss grade was grade 7. Specialist conditions: the anterior hairline and bilateral frontal horns moved up significantly, and only the core area, central posterior area, and top-rotation area had villous hair growth. , the scalp is visible, the hair on the back of the occiput is sparse and soft, and the scalp is visible. Diagnosis: Androgenetic Alopecia

[0059] After treatment:

[0060] On November 25, 2019, the patient was admitted to the hospital at 08:00. The blood routine and four infectious diseases were routinely checked before the operation, and contraindications to the operation were excluded; the scalp was cleaned routinely. Entered the operating room at 08:20, performed autologous hair follicle extraction (FUE) under local anesthesia, and completed the operation at 08:50. ...

Embodiment 2

[0074] Patient Li Fan, male, 28 years old, was admitted to the hospital due to "hair loss for many years". The hair loss grade was grade 7. The condition of the specialist: the front hairline and the bilateral frontal horns moved up significantly, and only the central posterior area and the top rotation area had villous hair growth. The scalp is visible, the posterior occipital hair is sparse and soft, and the scalp is visible. Diagnosis: Androgenetic Alopecia

[0075] After treatment:

[0076] On October 17, 2019, he was admitted to the hospital at 13:30. The blood routine and four infectious diseases were routinely checked before surgery to rule out contraindications to surgery; the scalp was cleaned routinely. Enter the operating room at 14:00, perform autologous hair follicle extraction (FUE) under local anesthesia at 14:00, and complete the operation at 14:30, extract 91 single hair follicles and 23 double hair follicles and put them into the nutrient solution reagent bo...

Embodiment 3

[0091] The patient, 51 years old, was admitted to the hospital due to "hair loss for many years". Hair loss grade: female is sparse and moderate, specialist condition: bilateral frontal horns move upward obviously, a little hair growth is seen, and the scalp is visible. The hair in the core area, central area and top-spin area is sparse and soft as a whole, and the scalp is visible. The hair on the back of the occiput is sparse and soft, and the scalp is visible. Diagnosis: androgenetic alopecia.

[0092] After treatment:

[0093] On December 4, 2019, the patient was admitted to the hospital at 09:30. The blood routine and four infectious diseases were routinely checked before surgery to rule out surgical contraindications; a small area of ​​​​the back of the occipital was routinely cut and the scalp was cleaned. Entered the operating room at 10:00, performed autologous hair follicle extraction (FUE) under local anesthesia, and completed the operation at 10:30. 61 single hai...

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Abstract

The invention discloses a hair follicle microarray co-culture system and application in treating pathological alopecia. The hair follicle microarray co-culture system comprises a hair follicle stem cell suspension and damaged hair follicles to be repaired. According to the invention, a new idea for creating a microenvironment suitable for hair follicle repair by combining allogeneic normal human hair follicle stem cells with drug molecules is provided, and the hair follicle microarray co-culture system for performing in-vitro 3D co-culture on hair follicle stem cells and damaged hair folliclesby using soft agar is provided.

Description

technical field [0001] The invention relates to the fields of genetic engineering technology and biomedicine, in particular to a hair follicle microarray co-cultivation system and its application in treating pathological alopecia. Background technique [0002] Hair follicle stem cells (HFSCs) are a group of multipotent stem cells capable of self-renewal and proliferation existing in the protuberance of the outer root sheath of hair follicles, which can differentiate into hair follicles, sebaceous glands, and epidermis, and play an important role in hair regeneration. effect. Studies have shown that human hair follicle stem cells express a variety of cell surface molecular markers such as CD200, keratin 15 (keratin 15, K15), keratin 19 (keratin 19, K19), integrin α6, integrin β1, Nestin, etc., but not Expression of endothelial cell marker molecule CD31; in addition, marker molecule CD34 of mouse hair follicle stem cells was not expressed. Using these positive and negative m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074A61K35/36A61K38/22A61P17/14
CPCC12N5/0628A61K35/36A61K38/22A61P17/14C12N2501/115C12N2509/00C12N2513/00C12N2533/76A61K2300/00
Inventor 周翔李胜华
Owner HUAKE TONGJI STEM CELL GENETIC ENG CO LTD
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