Skin culture medium for skin grafting operation of bioactive glass material and preparation method of skin culture medium

Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to a skin culture medium for a skin grafting operation of a bioactive glass material and a preparation method of the skin culture medium. The skin culture medium comprises a basal culture medium and a silver-loaded porous block bioactive glass culture medium. The skin culture medium also comprises the following components based on concentrations: 1-2ng / ml epidermal growth factors, 0.5-1.5 ng / ml transforming growth factor-beta, 1-3ng / ml fibroblast growth factor, 0.5-1ng / ml insulin growth factor, 1-3ng / ml keratinocyte growth factor, 1-2ng / ml vascular endothelial growth factor, 5-15ug / ml insulin, and 10-20ug / ml bovine pituitary extract. According to the method, the proliferation rate of epidermal tissue cells can be remarkably increased, the cell yield is high, and cell gene expression is effectively activated, so that the effect of promoting generation and growth of human tissue is achieved, good biocompatibility and surface activity are achieved, the bed surface regeneration and repair quality is effectively improved, besides, excellent antibacterial and bacteriostatic performance is achieved, the pollution opportunity introduced in the cell culture process is greatly reduced, and the safety is high.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a skin culture medium for skin grafting of bioactive glass materials and a preparation method thereof. Background technique [0002] The skin is the largest organ in the human body. It undertakes functions such as protecting the body, perspiration, and feeling cold, heat, and pressure, and protects various tissues and organs in the body from physical, mechanical, chemical, and pathogenic microorganisms. Genetic diseases, burns, chronic skin trauma (such as diabetes), vitiligo, albinism and many other reasons can cause damage to the skin, affect the appearance of the skin, leave scars on the skin and even lose its physiological function. When the skin is seriously damaged, such as deep and large-scale burns, the skin can no longer recover by itself, and will lose its resistance to bacteria, causing a large number of bacteria to invade the human body through the skin, ...

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a skin culture medium for skin grafting of bioactive glass materials and a preparation method thereof, which solves the problem of low proliferation rate and low cell yield in the existing cell culture medium for culturing cells. Not high defects, the promotion effect on the formation and growth of human tissue is small, and the antibacterial and antibacterial properties are poor, and it is easy to cause cell contamination during the cell culture operation, which brings harm to the wound skin graft repair

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