Micro-fluidic valve and micro-fluidic chip

A technology of microfluidic chips and microvalves, applied in the field of microfluidics, can solve the problems of increasing the sealing steps of finished chips, poor sealing performance, cumbersome process, etc., and achieve the effects of ensuring stability, small deformation, and simple preparation process

Inactive Publication Date: 2021-02-02
GUANGZHOU PLUSLIFE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both mechanical valves and pneumatic valves need to cover a layer of chips on the outside of the film, because the film is too thin and easy to break, and the air permeability leads to poor airtightness, and an additional layer of chip protection is required, which increases the sealing steps of the finished chip, and the process is cumbersome

Method used

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  • Micro-fluidic valve and micro-fluidic chip
  • Micro-fluidic valve and micro-fluidic chip
  • Micro-fluidic valve and micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 A microfluidic control valve

[0046] Including pipes and micro valves 6, one side 7 of the pipes is made of elastic materials such as pdms or transparent silicone, and the other side 8 of the pipes is made of hard materials such as pmma, resin materials, etc., and the two sides are bonded with double-sided tape.

[0047] The microvalve 6 is located inside the pipeline and is cylindrical. The cross-sectional width of the microvalve 6 is equal to the width of the pipeline. One end of the microvalve is fixedly connected to the pipeline, and its height is 90% of the pipeline height. When the external force is applied, the other end of the microvalve 6 is in contact with the pipeline, and the pipeline is closed to realize the closure of the liquid in the pipeline. When the external force is removed, the liquid in the pipeline resumes its flow.

Embodiment 2

[0048] Example 2 A microfluidic chip

[0049] Such as image 3 As shown, it includes a sequentially connected sample inlet 1, the first microfluidic control valve 2 of the reaction module, the first reaction chamber 3 of the reaction module, the second microfluidic control valve 4 of the reaction module, and the second reaction chamber of the reaction module. 5. Detection area, air outlet. The structure of the microfluidic control valve is the same as in Example 1.

[0050] The chip channel is 1000 microns deep and 600 microns wide.

[0051] (1) Chip production

[0052] Use resin materials to make the collection inlet 1, the first microfluidic control valve 2 of the reaction module, the first reaction chamber 3 of the reaction module, the second microfluidic control valve 4 of the reaction module, and the second reaction chamber 5 of the reaction module, The bottom structure of the chip that integrates the detection area and the air outlet.

[0053] The top layer of the c...

Embodiment 3

[0063] Example 3 A use case of a microfluidic chip for nucleic acid detection

[0064] When applied to nucleic acid detection, the present invention provides a microfluidic chip for nucleic acid detection, the structure of which is the same as in Example 2. This example is a microfluidic chip for detecting African swine fever virus (ASFV). The specific amplification primers and gRNA of the microfluidic chip are designed for African swine fever virus (ASFV).

[0065] The protruding microvalve design of the microfluidic chip ensures that the pipeline can be closed with only a small amount of deformation, ensuring the sealing of the pipeline, and preventing the liquid in the reaction chamber from volatilizing to other areas in the chip when it is heated, which will affect the experimental results. Especially in pipes with a large aspect ratio, the sealing effect is more significant.

[0066] The first reaction chamber is pre-loaded with lyophilized reagents for constant temperat...

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Abstract

The invention relates to the technical field of micro-fluidic control, in particular to a micro-fluidic control valve and a micro-fluidic chip. The micro-fluidic control valve comprises a pipeline anda micro-valve, the micro-valve is located in the pipeline, one end of the micro-valve is connected with the pipeline and is in a protruding shape, the height of the micro-valve is smaller than that of the pipeline, at least one side of the pipeline is made of an elastic material, and when external force is applied, the other end of the micro-valve makes contact with the pipeline, and the pipelineis closed. The micro-fluidic control valve and the micro-fluidic chip are simple in structure, small in size, few in chip assembling steps, easy to produce and low in cost, valve opening and closingcontrol can be achieved through elastic deformation of the material of the chip without additionally arranging a thin film, meanwhile, when the valve is closed, flowing interference on liquid in the pipeline is small due to the small deformation quantity of the pipeline, and wide application prospects are achieved.

Description

technical field [0001] The present invention relates to the field of microfluidic technology, in particular to a microfluidic control valve, and also relates to a microfluidic chip with the microfluidic control valve. Background technique [0002] Microfluidic control is the core of the microfluidic chip operation. The processes involved in the chip, such as sampling, mixing, reaction, and detection, must be completed in a controllable fluid. Therefore, the microfluidic control valve is one of the main components of the microfluidic system. First, microfluidics controls the fluid through a valve so that the fluid flows in a specified direction. [0003] At present, microfluidic control valves are divided into passive valves and active valves. Passive valves do not require external power or control, and the change of the valve state can be realized by using the change of the flow direction and pressure of the fluid itself. The main representatives are double-chip one-way val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027
Inventor 李翰林陈翀黄嘉恩
Owner GUANGZHOU PLUSLIFE BIOTECH CO LTD
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