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Primer group for quantitative detection of escherichia coli DNA on surface of urinary catheter

A technology for quantitative detection of Escherichia coli, which is used in a kit for rapid quantitative detection of Escherichia coli DNA on the surface of urinary catheters, a primer set for rapid quantitative detection of Escherichia coli DNA on the surface of urinary catheters, and a primer set for rapid quantitative detection of Escherichia coli DNA on the surface of urinary catheters. The field of bacteria DNA, to achieve the effect of easy operation

Pending Publication Date: 2021-01-29
SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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Problems solved by technology

[0004] At present, the main disadvantages of the traditional method for the quantitative detection of Escherichia coli (commonly known as E. coli, Gram-negative short bacilli) DNA on the surface of urinary catheters include the requirements for the microbiology laboratory, and the microbial culture period can be as long as 48 hours. Identification and Quantification

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  • Primer group for quantitative detection of escherichia coli DNA on surface of urinary catheter
  • Primer group for quantitative detection of escherichia coli DNA on surface of urinary catheter
  • Primer group for quantitative detection of escherichia coli DNA on surface of urinary catheter

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Embodiment Construction

[0020] The present invention is further described below.

[0021] LAMP (Loop-mediated isothermal amplification) is a new nucleic acid amplification molecular technology, in which a set of four (or six) different primers bind to six (or eight) different regions on the target gene, making it have Highly specific. The primer set consisted of two outer (F3 and B3) primers, two inner primers (forward inner primer (FIP) and backward inner primer (BIP)) and loop primers (loop forward and loop back). By using BstDNA polymerase with high displacement activity, the LAMP reaction can be simply carried out under isothermal conditions, and real-time quantitative monitoring of pathogenic bacteria can be realized by the commonly used turbidity meter at the bedside. The present invention determines the optimal primer sequence of the most common Escherichia coli in urinary tract infection through a large number of clinical experiments.

[0022] Each primer set consists of an outer primer pai...

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Abstract

The invention discloses a primer group for quantitative detection of escherichia coli DNA on the surface of a urinary catheter. The primer group consists of an outer primer pair F3 / B3, an inner primerpair FIP / BI and a loop primer pair loop F / loop B, the invention also relates to a rapid quantitative detection method adopting the primer group. The invention also relates to a rapid quantitative detection kit containing the primer group. The detection method and the kit disclosed by the invention are simple and convenient to operate, rapid, accurate and easy to popularize, have higher sensitivity, do not need expensive instruments, can be used in on-site pathogen and primary hospital detection, and have important significance for monitoring and controlling Escherichia coli on the surface ofa urinary catheter.

Description

technical field [0001] The invention belongs to the technical field of virus detection, in particular to a primer set for rapid quantitative detection of Escherichia coli DNA on the surface of a urinary catheter. [0002] The present invention also relates to a method for rapidly and quantitatively detecting Escherichia coli DNA on the surface of the urinary catheter by using the above-mentioned primer set. [0003] The invention also relates to a kit for rapidly and quantitatively detecting Escherichia coli DNA on the surface of a urinary catheter by using the primer set. Background technique [0004] At present, the main disadvantages of the traditional method for the quantitative detection of Escherichia coli (commonly known as E. coli, Gram-negative short bacilli) DNA on the surface of urinary catheters include the requirements for the microbiology laboratory, and the microbial culture period can be as long as 48 hours. identification and quantification. PCR analysis r...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/689C12Q1/10C12N15/11
CPCC12Q1/6851C12Q1/689C12Q2531/119C12Q2537/1376C12Q2563/107
Inventor 杨洁常庆沈丽娟侯剑刚彭晓琼钱玮王政平
Owner SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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