Microbial degradation material for flushing-free ecological closestool and preparation method of microbial degradation material
A technology of microbial degradation and toilet, which is applied in the field of microorganisms to achieve the effects of energy saving, reasonable formula and labor saving
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Embodiment 1
[0020] The cultivation method of biological agent:
[0021] (1) Incline culture of bacteria
[0022] Bacillus subtilis, Candida utilis, Aspergillus oryzae, Pediococcus lactis, Lactobacillus acidophilus, Bacillus pumilus, Bacillus cereus, Bacillus licheniformis, Acinetobacter ruckerii and fecal Alkaline bacteria were activated in the inclined plane, and then placed in suitable petri dishes for purification to obtain Bacillus subtilis strains, Candida utilis strains, Aspergillus oryzae strains, Pediococcus lactophilum strains, and Lactobacillus acidiformis, Bacillus pumilus, Bacillus cereus, Bacillus licheniformis, Acinetobacter ruckerii and Alcaligenes faecalis for subsequent use;
[0023] (2) Liquid culture of strains
[0024] The Bacillus subtilis bacterial classification obtained in step (1), Candida utilis bacterial classification, Aspergillus oryzae bacterial classification, Pediococcus lactis bacterial classification, Lactobacillus acidophilus bacterial classification, ...
Embodiment 2
[0032] Liquid culture method of bacteria:
[0033] The slant strain of Bacillus subtilis was inoculated in the sterilized liquid medium, and then cultured aerobically at a constant temperature of 28°C for 40 hours to obtain the Bacillus subtilis liquid; the slant strain of Candida utilis was inoculated in the sterilized utilis liquid medium, followed by aerobic culture at a constant temperature of 25°C for 36 hours to obtain the Candida utilis strain; Aspergillus oryzae was inoculated in a sterilized liquid medium, followed by aerobic culture at a constant temperature of 30°C 40 hours, to obtain Aspergillus oryzae bacteria liquid; Pediococcus lactis was inoculated in the sterilized liquid medium, and then cultured aerobically at a constant temperature of 28°C for 50 hours to obtain Pediococcus lactis bacteria liquid; Lactobacillus acidophilus was inoculated in the sterilized bacteria liquid culture medium, followed by aerobic culture at a constant temperature of 40°C for 40 ho...
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