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Tissue culture medium for lycoris, callus culture method and method for establishing lycoris regeneration system

A technology of callus and culture method is applied in the field of establishing a regeneration system of the genus Lycoris, which can solve the problems of low induction rate and small reproduction coefficient, and achieve the effects of improving reproduction coefficient, improving reproduction efficiency and solving the problem of low induction rate.

Active Publication Date: 2021-01-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, papers or patents have been published on using bulb discs as explants to induce clustered buds and establishing a tissue culture and rapid propagation technology system for Lycoris plants, but the induction rate is not high when using bulb discs as explants High, 1 / 4 or 1 / 6 of the bulb disc can generally induce 5-10 buds, and the reproduction coefficient is small, which shows that the induction rate is generally not high.

Method used

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  • Tissue culture medium for lycoris, callus culture method and method for establishing lycoris regeneration system

Examples

Experimental program
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Effect test

Embodiment 1

[0063] (1) The seeds were collected and sterilized on October 25, 2018. The mature fruit pods of Amaryllis chinensis were collected, put into seed bags, and brought back to the laboratory for future use.

[0064] Peel off the fruit pods to get Chinese Lycoris seeds. Select healthy, plump and uniform seeds, rinse with detergent for 40 minutes, and then rinse with running water for 2 hours. On the ultra-clean workbench, put the seeds in a sterilized glass bottle for the first disinfection: sterilize with 75% ethanol for 40s, rinse with sterile water 3 times, about 30s each time; then perform the second disinfection: use 0.1% HgCl 2 Treat for 60 minutes, wash with sterile water 5 times, and dry the moisture on the surface with sterile absorbent paper.

[0065] (2) Acquisition of seed embryos: place the sterile seeds on the inoculation tray, cut the seeds along the seed ridge direction of Amaryllis seeds with a scalpel, take out the embryos and keep them intact, as explants, sta...

Embodiment 2

[0074] (1) Harvesting and sterilization of seeds On November 15, 2018, mature fruit pods were collected in the nursery of this laboratory, put into seed bags, and brought back to the laboratory for future use.

[0075] Remove the fruit pods to get the suddenly laughing seeds. Select healthy, plump and uniform seeds, rinse with detergent for 45 minutes, then rinse with running water for 2 hours and 15 minutes. On the ultra-clean workbench, put the seeds in a sterilized glass bottle for the first disinfection: sterilize with 75% ethanol for 40 seconds, rinse with sterile water three times, about 40 seconds each time; then perform the second disinfection: use 0.1% HgCl 2 Treat for 65 minutes, wash with sterile water for 6 times, and dry the moisture on the surface with sterile absorbent paper.

[0076] (2) Acquisition of seed embryos: place the sterile seeds on the inoculation tray, cut the Hudixiao seeds longitudinally along the ridge direction of Amaryllis seeds with a scalpe...

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Abstract

The invention relates to the field of plant culture, in particular to a tissue culture medium for lycoris, a callus culture method and a method for establishing a lycoris regeneration system. The invention provides the tissue culture medium for lycoris. The tissue culture medium comprises an induction medium, and the induction medium takes an MS culture medium as a basic culture medium and furthercomprises the following content components of 20-25g / L of glucose, 7.0-7.5 g / L of agar, 2.0-2.5 mg / L of TDZ, 2.0-2.5 mg / L of 2,4-D and 0.5-2.0 mg / L of carbon nanotubes. The pH value of the inductionmedium is 5.5 to 6.0. By adopting the tissue culture medium provided by the invention, the inductivity can be improved.

Description

technical field [0001] The invention relates to the field of plant culture, in particular to a tissue culture medium for Lycoris, a callus culture method and a method for establishing a regeneration system of Lycoris. Background technique [0002] Lycoris Herb. belongs to Amaryllidaceae (Amaryllidaceae), a perennial herb. Bulbs ovoid, leaves in spring, leaves band-shaped, umbels with 4-6 small flowers; flowers yellow, red, white; perianth lobes recurled and shrunken. The flowering period is from July to August. The flower stems are tall and straight, the flower shape is beautiful, and the flower color is gorgeous. All species of this genus have high ornamental value and are known as "Chinese tulips". It has gradually become a new type of fresh cut flowers, garden ground cover and potted bulbous flowers. [0003] In the natural state, Lycoris plants are generally propagated by natural division of bulbs, but their reproduction coefficient is quite low. On average, one flow...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G24/10A01G22/35
CPCA01H4/008A01H4/001A01G24/28A01G24/15A01G24/10A01G22/35
Inventor 郑玉红张鹏翀顾永华韩福贵鲍淳松
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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