Method for detecting nanoscale plastic particles in animal biological sample

Abstract

The invention discloses a method for detecting nanoscale plastic particles in an animal biological sample, and relates to the field of biological monitoring by utilizing the animal biological sample and toxicological research of tissues and organs. The method comprises the following steps: collecting biological samples such as animal marine products, rodent experimental animal organs and the like,digesting and releasing plastic particles in the samples through alkali liquor, carrying out surface modification on the nano-plastic by utilizing the properties of the digested product, and extracting the nano-plastic by utilizing the effects of solid-phase adsorbent adsorption, coating coagulating sedimentation and the like; and finally, detecting the concentration of the plastic specific indication compound by using a Pyr-GC-MS combined platform, and converting the mass concentration of the indication compound into the mass of the nano plastic. The problems caused by different filter membrane apertures, filter membrane blockage and the like when plastic in a tissue sample is extracted through conventional membrane filtration are solved. A trace and ultra-trace pollution analysis meansis provided for research on health problems caused by nano-plastics and exposure risks of people. The method is simple, low in cost, high in benefit and wide in application range.

Description

technical field [0001] The invention relates to the field of biological monitoring and toxicology of tissues and organs, in particular to a detection method for nanoscale plastic particles in animal biological samples. Background technique [0002] Since the development of the plastic field in the middle of the 20th century, the world's plastic production has grown exponentially. Plastic products are widely used in various fields due to their advantages of low price and durability, especially the large increase in the use of disposable plastic products, which directly leads to Plastic waste is increasing year by year. Due to poor management of plastic waste, the ocean has become the final destination of most plastic waste. The large amount of plastic waste in the marine environment directly affects the survival and growth of marine life. In addition to large particles of plastic waste, nanoplastics that are produced through physical and chemical actions and can enter the b...

AI Technical Summary

Problems solved by technology

[0004] Despite the rapid development of plastic research in the past five years, and some studies have attempted to study the exposure of plastic particles from the level of biomonitoring, the detection technology is limited to characterizing micron-sized plastics, and the extraction and quantification methods used in various studies are different. First, the lack of comparability among various results ([9] Li P, et al. Analytical methods and environmental processes of nanoplastics. Environ. Sci. 2020; 94:88-99.), leading to poor promotion of various detection techniques, And there is a lack of qualitative and quantitative methods for nanoplastic particles in tissue samples

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