Micro-fluidic chip convenient for droplet tiling

Abstract

The invention discloses a micro-fluidic chip convenient for droplet tiling. The micro-fluidic chip comprises a chip body, a sealing layer and a plurality of PCR reaction units arranged on the chip body, each PCR reaction unit comprises a micro-channel arranged on the surface of the chip body, and a sample storage area, a first oil phase pool, a droplet generation area, a droplet transition area, adroplet tiling area and a collection area which are communicated with one another through the micro-channel; and the sealing layer is arranged on the surface of the chip body and is used for sealingthe micro-channel. Droplets are not directly tiled in the droplet tiling area during droplet generation, but are collected in the first oil phase pool firstly and then distributed into the droplet tiling area through the droplet transition area so that compared with the existing chip, the design solves the problem that the droplet tiling is not disturbed even if bubbles are generated in the droplet generation process, meanwhile, the problem of oil shortage in the droplet generation process is also solved.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip that facilitates flat laying of droplets. Background technique [0002] The existing micro-droplet digital PCR system mostly adopts a split technology route, that is, droplet generation, PCR reaction and droplet detection are completed on different instruments. Clinical diagnostic analysis requirements restrict the clinical application of this technology. With the continuous development of microfluidic chip technology, more and more people use microfluidic chips to realize the whole process of droplet generation, droplet storage and tiling, PCR thermal cycle and fluorescence imaging detection, etc., but the existing When the microfluidic chip is in operation, it basically generates droplets and spreads them at the same time, which is prone to lack of oil and bubbles, which will affect the detection accuracy, and at the same time, it is extremely in...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of microfluidic chip that is convenient for droplet tiling, this chip is not directly tiled in the droplet tiling area when the droplet is generated, but is first collected in the first oil phase pool, and then The droplet transition area is distributed to the droplet tiling area. Compared with the existing chip, this design solves the problem that even if bubbles are generated during the droplet generation process, it will not interfere with the droplet tiling. At the same time, it also solves the problem of Solved the problem of lack of oil in the droplet formation process

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