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Detection test strip based on quantum dot labeling as well as preparation method and application thereof

A technology of quantum dots and test strips is applied in the field of Salmonella detection test strips and their preparation, which can solve the problems of small quantity and efficient detection, and achieve the effects of low cost, simple operation and simple process.

Pending Publication Date: 2020-12-22
中华人民共和国太仓海关
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of food-borne pathogenic bacteria contaminated in food is usually relatively small, and conventional analysis methods are usually unable to perform rapid and efficient detection

Method used

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  • Detection test strip based on quantum dot labeling as well as preparation method and application thereof
  • Detection test strip based on quantum dot labeling as well as preparation method and application thereof
  • Detection test strip based on quantum dot labeling as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of test strips:

[0035] S1: First stick the NC film on the back plate, then stick the sample pad and absorbent paper on the upper and lower sides of the NC film respectively, covering the edge of the NC film by about 2mm;

[0036]S2: Take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin to modify the complementary chain of aptamer to obtain solution 1; take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin Poly A was modified to obtain solution 2, and solution 1 and solution 2 were placed in a shaker at 37°C and 180 RPM to incubate overnight, and the incubated conjugate solution 1 and conjugate solution 2 were respectively mixed at 1.0 The μL / cm spraying amount is sprayed on the T line and C line of the NC film to obtain the sprayed test paper;

[0037] S3: Dry the test paper sprayed in step S2 at 37°C for 2 hours to fix the T-line and C-line on the NC film, and finally use a strip cutter to cut the test paper into 4mm wide test strips and p...

Embodiment 2

[0041] Preparation of test strips:

[0042] S1: First stick the NC film on the back plate, then stick the sample pad and absorbent paper on the upper and lower sides of the NC film respectively, covering the edge of the NC film by about 3mm;

[0043] S2: Take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin to modify the complementary chain of aptamer to obtain solution 1; take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin Poly A was modified to obtain solution 2, and solution 1 and solution 2 were placed in a shaker at 37°C and 180 RPM to incubate overnight, and the incubated conjugate solution 1 and conjugate solution 2 were respectively mixed at 1.0 The μL / cm spraying amount is sprayed on the T line and C line of the NC film to obtain the sprayed test paper;

[0044] S3: Dry the test paper sprayed in step S2 at 37°C for 2 hours to fix the T-line and C-line on the NC film, and finally use a strip cutter to cut the test paper into 4mm wide test strips and ...

Embodiment 3

[0048] Preparation of test strips:

[0049] S1: First stick the NC film on the back plate, then stick the sample pad and absorbent paper on the upper and lower sides of the NC film respectively, covering the edge of the NC film by about 4mm;

[0050] S2: Take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin to modify the complementary chain of aptamer to obtain solution 1; take 50 μL 0.125 mg / mL streptavidin (SA) solution, add biotin Poly A was modified to obtain solution 2, and solution 1 and solution 2 were placed in a shaker at 37°C and 180 RPM to incubate overnight, and the incubated conjugate solution 1 and conjugate solution 2 were respectively mixed at 1.0 The μL / cm spraying amount is sprayed on the T line and C line of the NC film to obtain the sprayed test paper;

[0051] S3: Dry the test paper sprayed in step S2 at 37°C for 2 hours to fix the T-line and C-line on the NC film, and finally use a strip cutter to cut the test paper into 4mm wide test strips and ...

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Abstract

The invention discloses a salmonella detection test strip based on quantum dot labeling and a preparation method of the salmonella detection test strip and belongs to the field of food safety detection. A ZnS:Mn quantum dot is connected with a salmonella aptamer to serve as a signal probe, and the salmonella is detected by utilizing a test strip chromatography principle. The test strip is composedof a sample pad, a nitrocellulose membrane (NC film), absorbent paper and a back plate. the aptamer complementary sequence connected with streptavidin and Poly A is sprayed and fixed on a NC film byusing a BioDot three-dimensional spray point instrument to form a detection line T line and a quality control line C line. The quantum dots and the salmonella aptamer (the aptamer contains a Poly T sequence) are coupled to serve as a signal probe, and when the signal probe and a sample solution are mixed and then dropwise added to a sample pad, due to the capillary force effect caused by absorbentpaper, the signal probe migrates from the sample pad to the absorbent paper through the NC film. The salmonella is detected according to the fluorescence intensity ratio of the T line to the C line.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a salmonella detection test strip based on quantum dot marking and a preparation method and application thereof. Background technique [0002] Foodborne diseases have become a global public health problem, among which the incidence of foodborne diseases caused by foodborne pathogenic bacteria is the highest, posing a huge threat to human health. According to the "Notice of the National Health and Family Planning Commission on the National Food Poisoning Incidents in 2015", the number of people with microbial food poisoning is the largest, accounting for 53.7% of the total number of people with food poisoning throughout the year. About 300 million people in my country get sick every year due to Salmonella, accounting for 70-80% of the total foodborne diseases caused by pathogenic bacteria. Food poisoning incidents caused by foodborne pathogens are frequently repor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/58G01N33/558
CPCG01N33/56916G01N33/588G01N33/558G01N2333/255
Inventor 戴邵亮
Owner 中华人民共和国太仓海关
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