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Primer design and amplification method of Acanthogobio guentheri mitochondrial genome complete sequence

A mitochondrial genome and design method technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems that there are not many studies on eel gizzards, and the primers and methods for the full sequence amplification of mitochondrial genome have not been reported.

Inactive Publication Date: 2020-12-18
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at this stage, the research on ginobi is mainly concentrated in the field of proteomics and resource biology, while there are not many studies on ginobi using molecular biology techniques, and there are no primers and methods for the full sequence amplification of its mitochondrial genome. to report

Method used

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  • Primer design and amplification method of Acanthogobio guentheri mitochondrial genome complete sequence
  • Primer design and amplification method of Acanthogobio guentheri mitochondrial genome complete sequence
  • Primer design and amplification method of Acanthogobio guentheri mitochondrial genome complete sequence

Examples

Experimental program
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Effect test

Embodiment 1

[0090](1) Collection, identification and preservation of gargoyle samples

[0091]The samples used in the examples were all collected from June to July 2017 in the natural waters of the Yangqu River section of the Yellow River in the wild. All the samples were taken back to the laboratory for information registration, and were compared and dissected through morphological observation Identification to determine the thorny 鮈. After the species identification, the samples of the gargoyles were soaked in 100% absolute ethanol and stored in a refrigerator at 4°C for later use.

[0092](2) Extraction of mitochondrial genome

[0093]The phenol / chloroform method was used to extract the mitochondrial genomic DNA of the sample to be tested. The extracted total mitochondrial genomic DNA was stored at -20°C for use.

[0094](3) Design and synthesis of primers for mitochondrial gene sequence

[0095]Download the complete mitochondrial genome sequence (Romanogobio tenuicorpus, Saurogobio dabryi, Gobio cynocepha...

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Abstract

The invention relates to a primer design and an amplification method of a Acanthogobio guentheri mitochondrial genome complete sequence. The mitochondrial genome complete sequence of the Acanthogobioguentheri related species is obtained, a relatively conserved region and a region with larger variation are obtained by performing multiple comparison on the sequences, and mutually nested primers aredesigned in the relatively conserved region, namely, a downstream primer of a first pair of primers is behind an upstream primer of a second pair of primers, so that mutual overlapping of amplified products is ensured. The Acanthogobio guentheri mitochondrial genome complete sequence amplification primer can be used for variety identification, geographic population identification, germplasm resource evaluation and other aspects.

Description

Technical field[0001]The invention belongs to the field of fish mitochondrial genome research, and specifically relates to the primer design of the complete sequence of the mitochondrial genome of Pterocephalus and its amplification method.Background technique[0002]Acanthogobio guentheri Herzenstein (Acanthogobio guentheri Herzenstein) belongs to the order Cypriniformes, Cyprinidae, Subfamily, Acanthogobio, alias Goldfish (Qinghai Minhe), small fish, is the only species of Acanthogobio genus, and is a fish endemic to China. The body is tall and flat, the front of the dorsal fin is relatively bulging, the abdomen is round, and the tail stalk is thick and short. The head is short and pointed, and the head length is usually less than body height. The tip of the snout is tapered and the length of the snout is slightly shorter than the length of the head behind the eyes. The lower part of the mouth is curved, with simple lips and no papillae. The posterior sulcus is interrupted. The fina...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2600/156
Inventor 阙延福汪鄂洲李伟涛朱滨邵科廖小林闫书详胡兴坤
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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