Lactic acid bacteria enrichment culture medium suitable for industrial production and production process thereof

Abstract

The invention discloses a lactic acid bacteria enrichment culture medium suitable for industrial production and a production process thereof, and belongs to the technical field of microorganism culture media. According to the key technical points, the lactic acid bacteria enrichment culture medium is prepared from the following components in parts by weight of 400 to 430 parts of glucose, 400 to 430 parts of soybean meal zymolyte, 35 to 45 parts of growth factor, 5 to 15 parts of vitamins, 75 to 93 parts of buffer agent, 3.5 to 4.8 parts of magnesium sulfate and 0.5 to 1.5 parts of manganese sulfate, so that the culture cost of lactic acid bacteria in production is reduced. The use process of the lactic acid bacteria enrichment culture medium comprises the following steps of (1) adding water into a container, controlling the water temperature at 35 + / -2 DEG C, adding all the raw materials, and performing stirring to prepare an aqueous solution with the weight concentration of 1.8-2.5%;and (2) inoculating a powdery compound lactobacillus inoculant into the aqueous solution obtained in the step (1), and performing activating for 12 hours or above at the temperature of 35 + / -2 DEG Cfor use.

Description

technical field [0001] The application relates to the field of microbial culture medium, in particular to a lactic acid bacteria enrichment medium suitable for industrial production and a production process thereof. Background technique [0002] Lactic acid bacteria are widely distributed in nature and there are many kinds. Most of them are essential flora with important physiological functions for humans and animals. A variety of nutrients, adjust the pH value of the intestinal tract, and inhibit the reproduction of harmful microorganisms, so lactic acid bacteria have been widely used in the aquaculture industry. [0003] At present, most of the culture of lactic acid bacteria adopts MRS broth medium, which mainly uses peptone, beef, yeast powder, glucose, etc. Broth medium contains animal-derived components, which are generally not suitable for use in cattle feed. At the same time, the cost is too high when used in large quantities in production, resulting in a substantia...

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Problems solved by technology

[0003] At present, most of the culture of lactic acid bacteria adopts MRS broth medium, which mainly uses peptone, beef, yeast powder, glucose, etc. Broth medium contains animal-derived components, which are generally not suitable for use in cattle feed. At the same time, the cost is too high when used in large quantities in production, resulting in a substantial increase in the production cost of feed

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