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A system for studying mechanisms of immunosenescence in HIV-infected individuals

A technology for immune aging and infection, applied in the field of medical research, it can solve the problem that the mechanism and process of immune aging in HIV-infected patients cannot be completely and accurately reflected, the correlation and mutual influence of multiple indicators are not considered at the same time, and the HIV infection cannot be explained. Issues such as the immune aging process of infected patients

Active Publication Date: 2021-06-18
成都市疾病预防控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two indicators cannot explain the immune aging process in HIV-infected patients
[0004] In the existing technology, the research model on the immune aging mechanism of HIV-infected patients is still in the comparison and research of a single index, without considering the correlation and mutual influence between multiple indexes in the three dimensions of nucleic acid, cells, and cytokines at the same time , so the analysis results of the established research model cannot completely and accurately reflect the immune aging mechanism and process of HIV-infected patients

Method used

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  • A system for studying mechanisms of immunosenescence in HIV-infected individuals
  • A system for studying mechanisms of immunosenescence in HIV-infected individuals
  • A system for studying mechanisms of immunosenescence in HIV-infected individuals

Examples

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Comparison scheme
Effect test

Embodiment 1

[0057] Such as figure 1 A system shown for studying mechanisms of immune aging in HIV-infected individuals includes:

[0058] A sample collection unit for obtaining whole blood samples and plasma samples from HIV-infected persons;

[0059] The first detection unit is used to detect the content of the HIV-1 DNA virus library in the whole blood sample to obtain HIV-1 DNA detection data;

[0060] The second detection unit is used to detect the content of T lymphocyte subsets related to immune aging, and obtain the detection data of T lymphocyte subsets;

[0061] The third detection unit is used to detect the content of cytokines related to body inflammation and homeostasis in the plasma sample, and obtain cytokine detection data;

[0062] The analysis unit is used to receive and analyze the correlation and degree of mutual influence among HIV-1 DNA detection data, T lymphocyte subset detection data and cytokine detection data.

[0063] In some embodiments, the first detection un...

Embodiment 2

[0067] On the basis of Example 1, the first detection unit includes a quantitative standard curve drawing unit, and the quantitative standard curve drawing unit formulates a double-gene quantitative standard curve based on the internal reference gene of the human specific gene fragment and the specific gene of HIV-1 DNA to For PCR quantitative detection.

[0068] In one or more embodiments, the first detection unit includes a validity verification unit for double verification of the qualitative result. In one embodiment, the validity verification unit is also used to verify the validity of the kit and / or the validity of the sample.

Embodiment 3

[0070] On the basis of the above examples, the T lymphocyte subsets related to immune senescence include naive T lymphocytes, central memory T lymphocytes, effector memory T lymphocytes, activated T lymphocytes, senescent T lymphocytes, and aging T lymphocytes. one or more of cells, exhausted T lymphocytes, and thymus-exported T lymphocytes. Selecting T lymphocyte subsets as the research object, including cell subsets representing abnormal differentiation, overactivation, overaging, overaging, overexhaustion and weakened thymic output function, can more sensitively and comprehensively reflect the immune aging of T lymphocytes Case.

[0071] In one or more embodiments, the second detection unit uses a monoclonal antibody fluorescent dye to stain T lymphocyte subsets, wherein the fluorescent monoclonal antibody of naive T lymphocytes is CD3-PC7, CD4-FITC, CD8-PC5 .5. CD45RA-PE, CD27-ECD; fluorescent monoclonal antibodies of central memory T lymphocytes and effector memory T lym...

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Abstract

A system for studying the mechanism of immunosenescence of HIV-infected persons, the system comprising: a sample collection unit for obtaining whole blood samples and plasma samples of HIV-infected persons; a first detection unit for detecting HIV in the whole blood samples ‑1 The content of DNA virus library; the second detection unit is used to detect the content of T lymphocyte subsets related to immune aging; the third detection unit is used to detect the content of cytokines related to body inflammation and homeostasis in plasma samples The analysis unit is used to receive and analyze the correlation and the degree of mutual influence among HIV‑1 DNA, T lymphocyte subsets and cytokines. The present invention establishes a detection system and method covering nucleic acid, cells and cytokines, combined with statistical analysis to more completely reflect the mechanism and process of immune aging of HIV-infected patients as a whole, not only for the research and development of HIV drugs and the evaluation of drug effects or The effects of other interventions provide objective scientific support and are also conducive to the reconstruction of immune function in HIV-infected patients.

Description

technical field [0001] The invention relates to the field of medical research, in particular to a system and method for studying the immune aging mechanism of HIV-infected persons. Background technique [0002] With the development of medical level, the application of high-efficiency antiretroviral therapy (Antiretroviral therapy, ART) can effectively inhibit the replication of HIV (Human Immunodeficiency Virus, HIV), greatly reducing the morbidity and mortality of AIDS, but it cannot completely eliminate The latent HIV virus in the body. Long-term chronic viral infection can damage the normal immune function of the body, trigger chronic systemic inflammation, and lead to the occurrence of immunosenescence (Immunosenescence). Unlike immune degeneration, immune senescence is an adaptive process of continuous depletion and remodeling caused by chronic antigenic load. Immune senescence is not unique to HIV, and other viral chronic infections such as CMV and middle-aged and el...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851C12Q1/70G01N33/68G01N33/58G01N33/543G01N15/14
CPCC12Q1/6851C12Q1/703G01N15/14G01N33/543G01N33/581G01N33/6863G01N33/6869G01N2015/1006G01N2333/525G01N2333/5412G01N2333/5418G01N2800/7095C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 郭悦张燕琳李姗珊
Owner 成都市疾病预防控制中心
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