Method for generating cells of t cell lineage

A cell lineage and cell technology, applied in the field of T cell lineage generation, can solve problems such as the need for limited scale

Pending Publication Date: 2020-12-04
SUNNYBROOK RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While this progress is encouraging, the use of these methods to generate proT cells for therapeutic use is limited by the need for scale-up in clinical production and is not routinely produced in clinically usable large-scale cell numbers

Method used

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  • Method for generating cells of t cell lineage
  • Method for generating cells of t cell lineage
  • Method for generating cells of t cell lineage

Examples

Experimental program
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Embodiment 1

[0154] Materials and methods:

[0155] source of hematopoietic stem cells

[0156] Human umbilical cord blood (UCB) samples were obtained from consenting postpartum mothers, drawn by syringe and collected at In a blood bag set containing citrate phosphate glucose anticoagulant (Baxter Healthcare, Deerfield, Illinois). Within 24 hours of collection, UCB monocytes were isolated using Ficoll density centrifugation and pre-enriched for lineage-negative (Lin-) using the EasySep Human CD34 Positive Selection Kit (Stemcell Technologies, Vancouver, BC) according to the manufacturer's instructions. CD34 + cell. Lin - Anti-human CD38 for cells - APC and anti-human CD34 - PE mAb stained and CD34 sorted using a BD Biosciences FACSAria sorter (BD, San Jose, CA) + CD38 - / lo cells to isolate human HSPCs. As determined by post-sorting analysis, the purity of the sorted human HSPCs was greater than 99%. CD34 from some cord blood + Cells and all mobilized peripheral blood (mPB) der...

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Abstract

A method of generating cells of a T cell lineage is provided comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b)isolating cells of the T cell lineage. In one embodiment, the cells of the T-cell lineage are progenitor T cells or mature T cells. Compositions, kits and uses thereof are also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Application No. 62 / 630,497, filed February 14, 2018, the contents of which are incorporated herein by reference in their entirety. technical field [0003] The present application relates to methods, compositions and kits for generating cells of T cell lineage and uses thereof. In particular, the present application relates to methods, compositions and kits for generating progenitor cells and mature T cells and uses thereof. Background technique [0004] T cells are key mediators of adaptive immunity and can be used as therapeutic agents in immunotherapy against pathogens and cancer. Hematopoietic stem cell transplantation (HSCT) provides an effective treatment for a wide range of malignant and non-malignant conditions, but the conditioning regimens required prior to treatment result in delayed T cell recovery (Krenger et al., 2011). In contrast to m...

Claims

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Application Information

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IPC IPC(8): C12N5/0783A61K35/17C07K14/705C07K17/00C12N5/071C12N5/0735C12N5/0789
CPCC07K14/705C12N5/0636C12N5/0075C12N2506/45C12N2501/42C12N2506/11A61K2239/31A61K39/4611A61K39/464A61K2239/38A61K48/00C12N2501/599A61K35/17
Inventor J·C·阻尼加-普鲁科尔M·莫塔希亚米A·特罗特曼-格兰特
Owner SUNNYBROOK RES INST
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