Operating a mass spectrometer utilizing mass spectral database search

Abstract

Real-time search (RTS) for mass spectrometry is described. In one aspect, a mass spectrometer can identify a candidate peptide for a product ion spectrum by searching a mass spectral database. While executing the search of the mass spectral database, the elapsed search time can be monitored. If the elapsed search time of the identification of the candidate peptide is completed before reaching a maximum value, then the mass spectrometer can perform further actions.

Description

technical field [0001] The present disclosure relates to apparatus and methods for mass spectrometry, and more particularly, to a data dependent operation of a mass spectrometer using the results of a mass spectrometry database search. Background technique [0002] The current focus of biological mass spectrometry is the identification, quantification and structural elucidation of peptides, proteins and related molecules. In such experiments, it is often necessary or desirable to perform controlled fragmentation of certain ions (known as tandem or MSn mass spectrometry) to obtain product ions whose mass spectra provide highly useful for confirming identification or deriving information about the analyte of interest. Information about the structural details of the object. A common method of MSn mass spectrometry is called data dependent acquisition (DDA, alternatively called information dependent acquisition). The DDA technique utilizes data obtained in one mass analysis sc...

AI Technical Summary

Problems solved by technology

If the RTS takes a significant amount of time, fewer scans can be performed, resulting in lower throughput, fewer data points collected, and wasted samples

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