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Gastric cancer detection panel based on next-generation sequencing technology and application of gastric cancer detection panel

A second-generation sequencing technology and second-generation sequencing technology are applied in the field of gastric cancer detection panels, which can solve problems such as non-uniformity and achieve the effect of reducing costs.

Pending Publication Date: 2020-11-27
FUDAN UNIV SHANGHAI CANCER CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the heterogeneity of tumor cells, the mutations of tumor cells in a tumor tissue are also different, which has become a key problem in realizing precision medicine by finding mutant genes to use targeted drugs

Method used

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  • Gastric cancer detection panel based on next-generation sequencing technology and application of gastric cancer detection panel
  • Gastric cancer detection panel based on next-generation sequencing technology and application of gastric cancer detection panel
  • Gastric cancer detection panel based on next-generation sequencing technology and application of gastric cancer detection panel

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Experimental program
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Effect test

Embodiment

[0031] Step 1. Take a patient's cancer tissue and blood samples to extract DNA. The kits used are TIANGENTGuide Cells / Tissue Genomic DNA Kit and TIANGENT TGuide Large Volume BloodGenomic DNA Kit (1-3ml).

[0032] Step 2, DNA sample library preparation. First use the Bioruptor UCD-300 non-contact automatic ultrasonic breaker to randomly interrupt the DNA, ctDNA does not need this step. Take 400ng of DNA, dilute it to 50μl with nuclease-free water, transfer it to a 0.5mL Eppendorf LoBind Tube, mix well, centrifuge briefly and put it on ice for use. Place the sample: Place the centrifuge tubes symmetrically (if there is a single tube, add water to the empty tube to balance), tighten the rotor, place it on the ice box, and pre-cool for 1-2 minutes. 150 ~ 200bp ultrasonic break. Repeat the previous step of ultrasonic crushing, a total of 9 cycles, and end in about 90 minutes. The library was then prepared using the KAPA library construction kit.

[0033] Step 3, RNA probe prepa...

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Abstract

The invention belongs to the technical field of polygene detection of gastric cancer, and discloses a gastric cancer detection panel based on a next-generation sequencing technology. According to theinvention, important exon regions and a part of intron regions of 557 genes are enriched by using a gene probe hybridization method, and high-depth sequencing is carried out, so events such as gene mutation, copy number variation and the like having clear clinical correlation with the gastric cancer can be accurately detected; and a multi-gene screening list is made into a probe, and high-risk genes are detected in a targeted mode through DNA sequencing, so gene mutation with guiding significance for diagnosis and treatment can be detected more efficiently, accurate typing of tumor patients are realized, and meanwhile, a plurality of tumor patients can be helped to participate in clinical tests.

Description

technical field [0001] The invention belongs to the technical field of multi-gene detection of gastric cancer, and relates to a gastric cancer detection panel based on next-generation sequencing technology and an application thereof. Background technique [0002] Cancer is the leading cause of death and disability in the world today. Each tumor includes both genetic (germline) and tumor-specific (somatic) variation. In the past decade, high-throughput sequencing and bioinformatics technology have made great progress, and mutation information of a large number of germline and somatic genes has been collected. A large number of clinical practices have proved that the treatment of specific proteins that are mutated in cancer patients is effective. In order to achieve the goal of precision medicine, which is to give the right drug to the right patient, many laboratories have begun to apply next-generation sequencing technology in cancer genetic testing. 1. Gastric cancer is o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/156C12Q2600/118C12Q2600/106C12Q2531/113C12Q2535/122C12Q2537/165
Inventor 徐大志盛伟琪章真王亚农郭伟剑朱晓东黄丹邵志敏黄薇胡欣施锦绣裴雨晨
Owner FUDAN UNIV SHANGHAI CANCER CENT
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