Centrifugal separation device, mixed liquid separation and cultivation method

Abstract

The application provides a centrifugal separation device, comprising: a centrifuge tank for centrifuging a mixed liquid, the mixed liquid includes at least two monomer liquids; at least one liquid guide pipe, one end of the liquid guide pipe leads to the inner cavity of the centrifuge tank, and is used to It is used to introduce the mixed solution into the inner cavity of the centrifuge tank or export the mixed solution in the inner cavity of the centrifuge tank; at least one storage tank, the storage tank is connected to the centrifuge tank, the storage tank has a connection cavity and a storage cavity, and the connection cavity is connected to the storage cavity Between the inner cavity of the centrifuge tank, the storage cavity is used to store the monomer liquid separated from the mixed liquid; at least one switch assembly, which is connected to the storage tank, is used to control the conduction or blockage of the connection cavity; and at least one pipetting One end of the pipette leads to the storage cavity, and is used to lead out the monomer liquid in the storage cavity. The application also provides a method for separating and culturing the mixed solution. The centrifugal separation device provided by the application can improve the separation efficiency, purity and stability of the mixed liquid.

Description

technical field [0001] The present application relates to the technical field of centrifugal separation, in particular to a centrifugal separation device and a method for separating and cultivating a mixed liquid. Background technique [0002] Cell specific gravity separation technique is one of the most widely used cell separation techniques. At present, the current application status of similar technologies is almost done separately from cell separation, cell proliferation culture, cell washing and other technical operations, which greatly reduces the efficiency of cell preparation in vitro, and also greatly increases the possibility of isolated samples being contaminated by the outside world. . In the existing extremely limited integrated treatment technology of cell separation and cell proliferation and culture, there are many defects such as complex structure of consumables used for cell separation and culture, high standards of production materials, difficult manufact...

AI Technical Summary

Problems solved by technology

At present, the current application status of similar technologies is almost done separately from cell separation, cell proliferation culture, cell washing and other technical operations, which greatly reduces the efficiency of cell preparation in vitro, and also greatly increases the possibility of isolated samples being contaminated by the outside world.

In the existing extremely limited integrated treatment technology of cell separation and cell proliferation and culture, there are many defects such as complex structure of consumables used for cell separation and culture, high standards of production materials, difficult manufacturing process, and high production cost of products. The cell separation efficiency, Purity and stability also need to be further improved

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