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Preparation method of anti-aging bacillus fermentation product

A fermentation product, the technology of Bacillus, which is used in cosmetic preparations, preparations for skin care, and medical preparations containing active ingredients, etc. Handling convenient effects

Pending Publication Date: 2020-11-27
广州优科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortage of existing cosmetics aimed at alleviating mental stress, the present invention provides a preparation method using the fermentation product of Bacillus as an excitatory neurotransmitter inhibitor

Method used

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  • Preparation method of anti-aging bacillus fermentation product
  • Preparation method of anti-aging bacillus fermentation product
  • Preparation method of anti-aging bacillus fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bacillus fermentation product, the mass fraction of 2-amino-4-(ethylcarbamoyl)-butyric acid is 70%, the mass fraction of the mass fraction of inositol is 28.6%, and the balance is sodium glutamate , glutamic acid, and soluble impurities precipitated in cells.

[0031] The preparation method comprises the following steps:

[0032] Step 1, 2g glucose, 1g peptone, 0.5g yeast extract powder, 0.5g beef extract powder, 1g sodium chloride, 95g distilled water, and prepare 100g of culture solution with a volume of about 98mL; put the culture solution into a high-temperature sterilization pot Internal high temperature sterilization;

[0033] Step 2, the concentration of viable bacteria was 10 9 Add 2 mL of the Bacillus subtilis seed solution to 100 g of the culture medium, adjust the pH to 7.3, and culture at 27°C for 24 hours on a shaker with sufficient oxygen, then collect the cells by centrifugation to obtain 1.2 g of wet cells;

[0034] Step 3, put 1.2g of wet cells int...

Embodiment 2

[0038] A bacillus fermentation product, the mass fraction of 2-amino-4-(ethylcarbamoyl)-butyric acid is 70%, the mass fraction of the mass fraction of inositol is 28.6%, and the balance is sodium glutamate , glutamic acid, and soluble impurities precipitated in cells.

[0039] The preparation method comprises the following steps:

[0040] Step 1, 2g glucose, 1g peptone, 0.5g yeast extract powder, 0.5g beef extract powder, 1g sodium chloride, 95g distilled water, and prepare 100g of culture solution with a volume of about 98mL; put the culture solution into a high-temperature sterilization pot Internal high temperature sterilization;

[0041] Step 2, the concentration of viable bacteria was 10 10 Add 2 mL of Bacillus subtilis seed solution to 100 g of the culture medium, adjust the pH to 7.8, culture at 32°C on a shaker with sufficient oxygen for 24 hours, collect the cells by centrifugation, and obtain 2.3 g of wet cells;

[0042] Step 3, put 2.3g of wet cells into 46g of s...

Embodiment 3

[0046] A bacillus fermentation product, the mass fraction of 2-amino-4-(ethylcarbamoyl)-butyric acid is 70%, the mass fraction of the mass fraction of inositol is 28.6%, and the balance is sodium glutamate , glutamic acid, and soluble impurities precipitated in cells.

[0047] The preparation method comprises the following steps:

[0048] Step 1, 2g glucose, 1g peptone, 0.5g yeast extract powder, 0.5g beef extract powder, 1g sodium chloride, 95g distilled water, and prepare 100g of culture solution with a volume of about 98mL; put the culture solution into a high-temperature sterilization pot Internal high temperature sterilization;

[0049] Step 2, set the concentration of viable bacteria to 3×10 9 Add 2 mL of Bacillus subtilis seed solution to 100 g of the culture medium, adjust the pH to 7.5, and culture at 30°C for 24 hours on a shaker with sufficient oxygen, then collect the cells by centrifugation to obtain 2.8 g of wet cells;

[0050]Step 3, put 2.8g of wet cells int...

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Abstract

The invention discloses a preparation method of an anti-aging bacillus fermentation product. Thalli are used as a bioreactor to convert sodium glutamate to obtain the fermentation product, and the method specifically comprises thalli culture, thalli conversion and separating purification. According to the present invention, the shortage of the existing cosmetics taking mental stress relieving as the starting point can be solved, the bacillus fermentation product is adopted as the excitatory neurotransmitter inhibitor, and the excitatory neurotransmitter inhibitor is adopted to relieve mental stress so as to provide effects of skin improving and endocrine improving.

Description

technical field [0001] The invention relates to a preparation method of an anti-aging bacillus fermentation product, which belongs to the field of cosmetics. Background technique [0002] Cosmetic neurology refers to neurocognitive enhancement for non-medical purposes. In recent years, because of its wide audience and far-reaching influence, it has attracted more and more attention and discussion in the western society on the ethical issues it may bring. [0003] A large number of scientific studies have confirmed that the skin also has emotions, and the quality of its emotions is mainly affected by the nerves and cells of the skin. At the same time, the emotions of the skin directly affect the quality of the skin. Under the influence of external factors such as the environment and spirit, the skin tends to lose its self-regulation ability, and then produce positive or negative substances, causing the skin to lose control of emotions. When positive substances are produced,...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/44A61K8/34A61Q19/08A61P17/00A61P5/00
CPCA61K8/99A61K8/44A61K8/345A61Q19/08A61P17/00A61P5/00A61K2800/85
Inventor 徐梦漪林朝栋卢晓菲
Owner 广州优科生物科技有限公司
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