Full-slide fluorescence hyperspectrum rapid acquisition method and device

A technology of fluorescence spectrum and acquisition method, which is applied in the field of fast acquisition of full-glass fluorescence hyperspectral spectrum, can solve problems such as affecting the quality of measurement images, and achieve the effects of avoiding mechanical vibration, high speed, and avoiding time expenses.

Inactive Publication Date: 2020-11-24
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

[0003] The traditional fluorescence detection method is mainly based on the design of the filter. The switching of the filter takes time and the process of switching the filter on the electronic control platform is accompanied by mechanical vibration, which affects the quality of the measurement image.

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  • Full-slide fluorescence hyperspectrum rapid acquisition method and device
  • Full-slide fluorescence hyperspectrum rapid acquisition method and device

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Embodiment Construction

[0031] Embodiments of the present invention will be described in detail below. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0032] It should be noted that when an element is referred to as being “fixed” or “disposed on” another element, it may be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element. In addition, connection may be for both a fixed function and a circuit / signal communication function.

[0033] It is to be understood that the terms "length", "width", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationships sh...

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Abstract

The invention discloses a full-slide fluorescence hyperspectrum rapid acquisition method and device. Exciting light emitted by the exciting light source is modulated into slit exciting light by the slit exciting light modulation device, the slit exciting light is projected to the surface of a sample on the glass slide to excite fluorescence, the fluorescence is dispersed by the prism, and a fluorescence spectrum image of a first camera pixel position after dispersion is acquired by the camera; the slit exciting light is translated by one pixel position, and a fluorescence spectrum image of thenext pixel position is obtained until images of all pixel positions in the current view field are acquired; the objective table is moved to enable the microscope objective lens to be aligned with theposition of the next view field of the glass slide, and the fluorescence spectrum image is repeatedly collected until the images of all view fields of the glass slide are collected; and the fluorescence spectrum images of all the pixel positions are combined in each view field to obtain a fluorescence spectrum image of each view field, and the spectrum images of all the view fields are spliced toobtain a fluorescence spectrum image of the whole glass slide. According to the invention, the fluorescence hyperspectral image of the whole glass slide can be rapidly obtained with high quality.

Description

technical field [0001] The invention relates to the field of spectrum collection, in particular to a method and device for rapid acquisition of full-slide fluorescence hyperspectrum. Background technique [0002] Hyperspectral or hyperspectral images have two spatial dimensions and one spectral dimension, which can provide information about tissue physiology, morphology, and composition, and are widely used in disease diagnosis and image-guided surgery. Existing spectral image acquisition methods include whiskbroom, pushbroom, staring, snapshot, etc. [0003] The traditional fluorescence detection method is mainly based on the design of the filter. The switching of the filter takes time and the process of switching the filter on the electronic control platform is accompanied by mechanical vibration, which affects the quality of the measurement image. Contents of the invention [0004] The main purpose of the present invention is to overcome the defects of the prior art, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N2021/6417
Inventor 张永兵辛开发季向阳王好谦
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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