Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for constructing a mouse model of spontaneous lung squamous cell carcinoma

A technology for constructing methods and mouse models, which can be applied to other methods of inserting foreign genetic materials, chemical instruments and methods, and botanical equipment and methods, etc., and can solve the problem of animals that are not specific for lung squamous cell carcinoma and lack oncogenes Impure models, histopathological types of spontaneous tumors, etc.

Active Publication Date: 2022-03-22
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently existing mouse models of lung squamous cell carcinoma, which have many obvious defects, mainly: (1) spontaneous tumor formation is extremely slow, often taking several months or even a year, which is very unfavorable for experimental research; (2) pathological changes in spontaneous tumor tissue The type is not pure, and there are multiple pathological subtypes in the same tumor tissue; (3) Since the knockout of tumor suppressor genes does not have the specificity of lung squamous cell carcinoma, etc., there is a lack of research on animal models that introduce oncogenes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for constructing a mouse model of spontaneous lung squamous cell carcinoma
  • A method for constructing a mouse model of spontaneous lung squamous cell carcinoma
  • A method for constructing a mouse model of spontaneous lung squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Design and construction of conditional Yap1 knock-in targeting vector: A mouse Yap1 conditional knock-in model was established in C57BL / 6 mice. (See figure 1 and figure 2 )

[0055] 1. Vector construction:

[0056] (1) Order BAC, and prepare the primers required for vector construction; (2) Extract BAC by shaking the bacteria, and prepare the basic vector plasmid (KI431-basic vector); (3) PCR amplify the target fragment Yap1 from BAC DNA, and detect the amplification by electrophoresis. Amplify the product, cut the gel to recover the target fragment; (4) connect the target fragment to the basic vector (cut the basic vector with pmlI and KpnI); (5) transform the ligated product into a competent state, spread it on a plate and culture it overnight; (6) pick a single colony , positive clones were screened by colony PCR; (7) small plasmids were extracted from colony PCR positive clones, and identified by enzyme digestion; (8) positive clones identified by enzyme dige...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for constructing a spontaneous lung squamous cell carcinoma mouse model, and relates to the technical field of mouse model construction; the present invention constructs a conditional Yap1 knock-in mouse (Yap1 KI ), due to the tumor-promoting effect of Yap1, the spontaneous lung squamous cell carcinoma has the characteristics of rapid tumor formation, and then according to the standard design of transgenic mice, a negative selection marker (DTA) and a positive selection marker (Neo) are also introduced into the targeting vector, It is convenient to quickly obtain positive clones, and finally use Trp53 gene knockout mice and Yap1 KI Mice were crossed to obtain Yap1 KI / Trp53 KO Mice, completed the construction of spontaneous lung squamous cell carcinoma mouse model.

Description

technical field [0001] The invention belongs to the technical field of mouse model construction, and in particular relates to a method for constructing a spontaneous lung squamous cell carcinoma mouse model. Background technique [0002] Lung cancer is currently the largest malignant tumor in human beings, and its morbidity and mortality both occupy the first place. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer patients; 60-80% of NSCLC is lung adenocarcinoma, 20-40% is lung squamous cell carcinoma, and for lung cancer, especially lung squamous cell carcinoma Animal models are essential for cancer treatment and mechanism research. However, there are currently existing mouse models of lung squamous cell carcinoma, which have many obvious defects, mainly: (1) spontaneous tumor formation is extremely slow, often taking several months or even a year, which is very unfavorable for experimental research; (2) pathological changes in spontaneous tumor tissu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/90C12N15/12A01K67/027
CPCC12N15/8509C12N15/907C07K14/82A01K67/0278C12N2800/107A01K2207/15A01K2217/072A01K2227/105A01K2267/03
Inventor 孙建国李奉孟令鑫廖星芸赵先兰余永新廖荣霞
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com