Pancreatic cancer detection kit and detection method (PCR method)

Abstract

The invention discloses a pancreatic cancer detection kit. The kit comprises a PCR reaction solution, polymerase, a negative control sample and a positive control sample, wherein the PCR reaction solution comprises dNTPs, PCR Buffer, a primer and a probe, the volume and the quantity of the raw materials are 0.96 mL x 2 parts respectively, the polymerase is Taq DNA polymerase, and the volume and the quantity of the raw materials are 85 [mu]l x 1 part respectively. The invention relates to the technical field of gene engineering. According to the pancreatic cancer detection kit and the detectionmethod (PCR method), pancreatic non-cancerous tissue and cancerous tissue can be distinguished through a DNA methylation state, the sensitivity and specificity of cancer tissue diagnosis are both 100%, and it can be found as soon as possible that secreted protein acidic and rich in cysteine (SPARC) of a recurrent patient plays a role in regulating the cell matrix effect and tumor angiogenesis, proliferation and migration. SPARC gene methylation occurs in the early stage of pancreatic cancer, so that detection of SPARC gene methylation is of great significance to early diagnosis of adenocarcinoma.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a pancreatic cancer detection kit and a detection method (PCR method). Background technique [0002] In recent years, the incidence of pancreatic cancer has been increasing year by year. Most patients have already metastasized when they are diagnosed, and the opportunity for radical surgery is lost, and the prognosis is extremely poor. Therefore, it is urgent to study the molecular mechanism of the occurrence and development of pancreatic cancer in depth, and to develop new ways for early diagnosis and treatment of pancreatic cancer. Research has shown that many tumors begin with epigenetic alterations and genetic mutations that initiate tumor-promoting processes. Epigenetic changes are heritable changes in gene phenotype without changes in the DNA sequence of the gene. DNA methylation is one of the important ways of gene epigenetics. [0003] Based on this, we ad...

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a pancreatic cancer detection kit and detection method (PCR method), which has the advantages of early diagnosis of pancreatic cancer and solves the problem of late diagnosis cycle of existing pancreatic cancer