Mouse liver organoid model, establishing method therefor and application of mouse liver organoid model

Abstract

The invention provides a mouse liver organoid model, an establishing method therefor and application of the mouse liver organoid model. The establishing method comprises the following steps: withdrawing liver tissue of a mouse, and carrying out cleaning, shearing, digesting and filtering treatment sequentially, so as to obtain liver stem cells of the mouse; and subjecting the liver stem cells of the mouse to primary culture, propagation culture and differentiation culture sequentially, thereby obtaining the mouse liver organoid model. The liver organoid model obtained by employing the method is applied to pharmacologic, pharmacodynamic and toxicologic analysis. Compared with the conventional methods, the liver organoid model establishing method provided by the invention has the advantagesthat on one hand, the culture cycle is shortened greatly: the time cycle from material obtaining to model obtaining is about 14 to 20 days, the consumed time of the conventional culture method is 30 days, and thus, the feasibility of model application is improved obviously; and on the other hand, by the method provided by the invention, the success rate of mouse liver organoid culture is increasedobviously, and the growth speed of organoids of primary culture is accelerated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a mouse liver organoid model and its establishment method and application. Background technique [0002] Drug-induced hepatotoxicity is currently the most critical safety indicator for new drugs. Immortalized hepatic cell lines and animal models are still the "gold standard" for drug metabolism and toxicity testing, but their practicability and long-term culture capacity are very limited, which greatly affects The breadth and depth of research are limited. Liver toxicity experiments based on animal models are costly and have a long experiment cycle, making rapid high-throughput detection and analysis impossible. In vitro models capable of high-throughput analysis are urgently needed for drug efficacy and toxicity testing in hepatotoxicity studies and preclinical drug development. Mice are a commonly used model for drug safety evaluation. Establishing a mouse liv...

AI Technical Summary

Problems solved by technology

[0002] Drug-induced hepatotoxicity is currently the most critical safety indicator for new drugs. Immortalized hepatic cell lines and animal models are still the "gold standard" for drug metabolism and toxicity testing, but their practicability and long-term culture capacity are very limited, which greatly affects Limits the breadth and depth of research

Liver toxicity experiments based on animal models are costly, long-term, and unable to perform rapid and high-throughput detection and analysis

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