Method for identifying and quantifying anaerobic iron ammonia oxidation path in reservoir sediment

A technology for anaerobic iron ammonia oxidation and path identification, applied in the field of identification and quantification of anaerobic iron ammonia oxidation path in reservoir sediment, can solve the problem of difficult quantitative evaluation of anaerobic iron ammonia oxidation activity

Active Publication Date: 2020-11-17
GUANGDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to provide a method for identifying and quantifying the anaerobic iron ammonium oxidation pathway in the reservoir sediment, aiming at the problem that the existing anaerobic iron ammonium oxidation activity is difficult to quantitatively evaluate

Method used

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  • Method for identifying and quantifying anaerobic iron ammonia oxidation path in reservoir sediment
  • Method for identifying and quantifying anaerobic iron ammonia oxidation path in reservoir sediment
  • Method for identifying and quantifying anaerobic iron ammonia oxidation path in reservoir sediment

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Embodiment

[0093] This embodiment provides a 15 N-NH 4 + The method for marking and tracing the ammonia oxidation activity of anaerobic iron, the specific operation steps are as follows:

[0094] S1. Sediment pre-cultivation

[0095] Weigh 2-3g of sediment samples into ten 12mL Labco glass culture bottles, put two glass beads with a diameter of 3mm in each, divide the culture bottles into two groups on average, and mark them as control group and sample test group 1 respectively. In the control group, the needles were filled with in-situ water pre-aerated with high-purity nitrogen to ensure the same concentration of organic carbon sources and Fe(III) as in the natural sampling environment. while the test group is filled with pre-C 2 h 2 Aerated in situ water, which specifically inhibits heterotrophic denitrification and anammox activity, allowing accurate determination of anaerobic iron ammonium oxidation activity. Cover the original rubber stopper well, and there is no air bubble i...

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Abstract

The invention provides a method for identifying and quantifying an anaerobic iron ammoxidation path in reservoir sediment. According to the invention, a method for inhibiting heterotrophic denitrification and anaerobic ammonia oxidation activity by using a 15N-NH4+ marker and C2H2 is adopted, and anaerobic iron ammonia oxidation reaction activity is calculated through comparative analysis of a test group and a control group; and the activity of anaerobic ammonia oxidation, heterotrophic denitrification and co-denitrification can be further determined by adding other marked test groups. According to the method disclosed by the invention, the activity of the Feammox bacteria can be determined, and the biological activity of other types of denitrification technologies can be accurately distinguished, so that a reliable evaluation method is provided for systematically knowing, regulating and controlling a biological denitrification path.

Description

technical field [0001] The invention belongs to the field of surface drinking water treatment, and in particular relates to a method for identifying and quantifying anaerobic iron ammonium oxidation paths in reservoir bottom mud. Background technique [0002] A healthy cycle of nitrogen plays an important role in maintaining the stability and balance of Earth's ecosystems. However, human exploitation and utilization of mineral energy and excessive application of nitrogen-containing fertilizers have seriously accelerated the input of nitrogen into the environment, leading to serious nitrogen pollution in the atmosphere and water. Current traditional theory (Tian T, Yu H. Denitrification with non-organic electron donor for treating low C / N ratio wastewaters[J]. Bioresource Technology. 2020, 299: 122686. Zhang Z, Zhang Y, Chen Y. Recent advances in partial denitrificationin biological nitrogen removal:From enrichment to application[J].BioresourceTechnology.2020,298:122444.) be...

Claims

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Application Information

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IPC IPC(8): C02F3/28G01N33/24C02F101/16
CPCC02F3/286G01N33/24C02F2101/16
Inventor 李宁江进许燕滨潘汉平关朝婷胡颖斌曹节
Owner GUANGDONG UNIV OF TECH
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