Construction and use of an animal model of senile constipation

An animal model and constipation technology, which can be used in compound screening/testing, preparations for in vivo tests, pharmaceutical formulations, etc., can solve the problems of high one-sidedness, poor flexibility, and inability to simulate constipation models well, and achieve strong specificity Effect

Active Publication Date: 2022-07-19
SHANXI UNIV
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Problems solved by technology

[0007] In order to solve the technical problems that the existing constipation model cannot better simulate the state of clinical senile constipation and the evaluation method of the constipation model is highly subjective, one-sided, and poor in flexibility, the present invention provides the construction and application of an animal model of senile constipation

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  • Construction and use of an animal model of senile constipation
  • Construction and use of an animal model of senile constipation
  • Construction and use of an animal model of senile constipation

Examples

Experimental program
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Effect test

Embodiment 1

[0062] Embodiment 1: a kind of construction method of senile constipation animal model, comprising the following steps:

[0063] D-galactose solution 300 mg / kg was subcutaneously injected at 8:00 a.m. every day, that is, the concentration was 300 mg / mL (0.1 mL / 100 g). From the 7th week, the first dose of 5 mL / rat was given white vinegar, and then the rats were alternately given white vinegar (3 mL / 200g) and 16.67 mol / L activated carbon ice-water mixture (5 mL / 200g) on ​​odd and even daily basis for 5 consecutive Weeks later, an animal model of senile constipation was obtained. The modeling time was 11 weeks in total.

[0064] The rats in the blank control group were constructed by subcutaneously injecting the same amount of normal saline as the model group at 8 am every day. From the 7th week, the same amount of distilled water was given to the model group, and the modeling time was 11 weeks in total.

Embodiment 2

[0065] Example 2: Validating the constructed animal model of senile constipation, applying multivariate statistical analysis method to characterize the metabolic profile, using principal component analysis method to perform metabolic profile analysis on the data, and examining the separation of each group of data.

[0066] The specific method is to collect the feces of the rats in the blank control group and the model group after the establishment of the animal model, and perform NMR analysis on them to obtain the NMR spectra of the rats in the blank control group and the model group. The results are shown in figure 1 and figure 2Then, multivariate statistical analysis was carried out on the integral data matrix of NMR spectra of the rats in the blank control group and the model group, and the contour maps of the endogenous metabolites in the feces of the rats in the blank control group and the model group were obtained. The results are shown in image 3 ,from image 3 It c...

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Abstract

The invention belongs to the technical field of model construction and verification, and provides a senile constipation animal in order to solve the technical problems that the existing constipation model cannot well simulate the state of clinical senile constipation and the evaluation method of the constipation model is highly subjective, one-sided and poor in flexibility. The construction of the model and its purpose. The rats in the model group were subcutaneously injected with D-galactose every day. From the 7th week, white vinegar was given by gavage at the first dose of 5 mL / rats. After that, white vinegar and activated carbon ice-water mixture were alternately fed on odd and even days. An animal model of constipation. Subcutaneous injection of D-galactose can induce aging, and gavage of white vinegar and activated carbon ice-water mixture can change gastrointestinal motility and cause constipation symptoms. It not only reflects the two characteristics of aging and constipation, but also reflects the characteristics of clinical senile constipation, simulates the clinical symptoms of constipation in the elderly, is suitable for the screening of traditional Chinese medicines for the treatment of senile constipation, and provides an experimental basis for the development of new drugs related to development.

Description

technical field [0001] The invention belongs to the technical field of model construction and verification, and in particular relates to the construction of an senile constipation animal model and its application. Background technique [0002] Constipation (Constipation) is a common digestive tract disease in the elderly, which can appear alone, as a concomitant symptom of some diseases, or become the inducement of other diseases. The prevalence of chronic constipation increases with age. According to the survey, the incidence of constipation in the elderly aged 60 to 64 years was 8.7%, and the incidence of constipation in the elderly aged ≥85 years was as high as 19.5%. Chronic constipation that is not cured for a long time not only makes the elderly too mentally stressed, but also is closely related to the occurrence of diseases such as lower gastrointestinal tumors, senile mental disorders and colon cancer. Therefore, senile constipation is a potentially harmful disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/20A01K2227/105A01K2267/03
Inventor 刘晓节赵迪赵思俊李震宇张丽增秦雪梅
Owner SHANXI UNIV
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