Conductive hydrogel biomimetic gradient mineralization method based on graphene bioactive macromolecules

A conductive hydrogel and bioactive technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of difficult hard tissue repair and reconstruction, poor biomechanical properties, etc., and achieve easy control of process parameters and convenient operation , low cost effect

Inactive Publication Date: 2020-11-17
苏州宣医智慧医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its biomechanical properties are poor, and it is difficult to apply to the repair and reconstruction of hard tissues. Therefore, it is necessary to add mechanically enhancing materials with certain bioactivity to the hydrogel in order to use hydrogel to construct bone / cartilage interface tissue engineering scaffolds. the goal of

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  • Conductive hydrogel biomimetic gradient mineralization method based on graphene bioactive macromolecules

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Embodiment 1

[0024] see figure 1 , the present invention provides a technical solution: a method for biomimetic gradient mineralization of conductive hydrogel based on graphene bioactive polymers, comprising the following steps,

[0025] Prepare 0.167 mol / L calcium nitrate solution, add 2-5% (w / v) agar to the mixture and stir at 95°C for 30 minutes.

[0026] Cool the solution prepared in S1 to 60°C, then add 0-3% (w / v) gelatin, and stir for 30 minutes.

[0027] Continue to add 0-0.5 mg / ml graphene oxide to the solution prepared in S1 under stirring at 60°C, stir for 30 minutes, and then quickly cool it to 5°C in an ice-water mixture to obtain agar / gelatin with calcium ions chelated / Graphene oxide hydrogel, graphene-enhanced agar / gelatin hydrogel can be biomimetic mineralized inside to form a physical and chemical gradient change structure, wherein the mass fraction of graphene is 0-1% by weight percentage, graphene, It can be replaced by any combination of one or more of other graphene ...

Embodiment 2

[0032] see figure 1 , the present invention provides a technical solution: a method for biomimetic gradient mineralization of conductive hydrogel based on graphene bioactive polymers, comprising the following steps,

[0033] S1: Prepare 0.167 mol / L calcium nitrate solution, add 5% (w / v) agar to the mixture and stir at 95°C for 30 minutes.

[0034] S2: Cool the solution prepared in S1 to 60°C, then add 3% (w / v) gelatin, and stir for 30 minutes.

[0035] S3: Continue to add 0.5mg / ml graphene oxide to the solution prepared in S1 under the condition of stirring at 60°C, stir for 30 minutes, and then quickly cool it to 5°C in the ice-water mixture to obtain agar / gelatin with calcium ions chelated / Graphene oxide hydrogel.

[0036] S4: The hydrogel obtained in S3 was adjusted to 0.2 mol / L (NH 4 ) 2 HPO 4 solution, and adjusted its pH value at 11 with ammonia water, and the reaction system was biomineralized at 37°C for 7 days.

[0037] S5: slice the material obtained in S4, t...

Embodiment 3

[0039] see figure 1 , the present invention provides a technical solution: a method for biomimetic gradient mineralization of conductive hydrogel based on graphene bioactive polymers, comprising the following steps,

[0040] S1: Prepare 0.167 mol / L calcium nitrate solution, add 2% (w / v) agar to the mixture and stir at 95°C for 30 minutes.

[0041] S2: Cool the solution prepared in S1 to 60°C, then add 1% (w / v) gelatin, and stir for 30 minutes.

[0042] S3: Continue to add 0.25mg / ml graphene oxide to the solution prepared in S1 under the condition of stirring at 60°C, stir for 30 minutes, and then quickly cool it to 5°C in the ice-water mixture to obtain agar / gelatin with calcium ions chelated / Graphene oxide hydrogel.

[0043] S4: The hydrogel obtained in S3 is adjusted to 0.1mol / L (NH 4 ) 2 HPO 4 solution, and adjust its pH value to 10 with ammonia water, and then put the reaction system at 37°C for biomineralization reaction for 2 days.

[0044] S5: slice the material...

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Abstract

The invention discloses a conductive hydrogel biomimetic gradient mineralization method based on graphene bioactive macromolecules. The method comprises the following steps that a calcium nitrate solution is prepared, and agar is added into the mixed solution and stirring is conducted at high temperature of 95 DEG C for 30 minutes; the solution prepared in the step S1 is cooled to 60 DEG C, and then gelatin is added and stirring is conducted for 30 minutes; the solution prepared in the step S1 is continued to be stirred at the temperature of 60 DEG C, graphene oxide is added and stirring is conducted for 30 minutes, and then the mixture is putted into an ice-water mixture to be quickly cooled to 5 DEG C to obtain agar / gelatin / graphene oxide hydrogel chelated with calcium ions; and the hydrogel obtained in S3 is putted into a (NH4) 2HPO4 solution, the pH value of the mixture is adjusted with ammonia water, and a biomineralization reaction is conducted on a reaction system at 37 DEG C for 2-7 days. The conductive hydrogel biomimetic gradient mineralization method based on the graphene bioactive macromolecules has the advantages that equipment is simple, the cost is low, the operationis convenient, the process parameters are easy to control and the like, and has a certain application value in the field of interface tissue engineering.

Description

technical field [0001] The invention relates to the technical field of preparation of tissue-engineered bone / cartilage biomimetic integrated scaffold for tissue repair and reconstruction of bone / cartilage interface, in particular to a biomimetic gradient mineralization method of conductive hydrogel based on graphene bioactive polymer. Background technique [0002] With the aging of the social population and the increase of sports injuries, the incidence of bone and joint lesions is increasing. In the treatment of traumatic bone / cartilage defects and degenerative joint diseases, it is difficult to firmly heal between bone tissue and cartilage tissue is an important problem in the field of bone tissue engineering. Therefore, construct functional biomimetic gradient bone tissue engineering scaffolds at the bone / cartilage interface, so that the chemical composition and physical structure of the material can match the biomimetic gradient properties of the interface tissue to be r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/08A61L27/56A61L27/22A61L27/20
CPCA61L27/08A61L27/20A61L27/222A61L27/52A61L27/56A61L2430/02A61L2430/06C08L5/12
Inventor 鲍路姿丁玉川冯磊黄弢
Owner 苏州宣医智慧医疗科技有限公司
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