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Synchronous detection device combining immunodetection with leukocyte counting and detection method thereof

A white blood cell counting and immune detection technology, which is applied in the field of analysis and detection, can solve the problems of low detection accuracy, leukocyte lysis, short detection time window, etc., and achieve the effects of improving detection efficiency, convenient portability, and simple structure

Pending Publication Date: 2020-11-10
苏州中科苏净生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hemolysis counting method adopted by HemoCue has the following defects: first, this method uses the transmission absorption method, which is easily interfered by impurities; second, the detection time window is short and must be strictly controlled within 2-10 minutes, as the time is too short to lyse red blood cells. Completely, some white blood cells will also be dissolved if the time is too long; the above two points lead to low detection accuracy of the device

Method used

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  • Synchronous detection device combining immunodetection with leukocyte counting and detection method thereof
  • Synchronous detection device combining immunodetection with leukocyte counting and detection method thereof
  • Synchronous detection device combining immunodetection with leukocyte counting and detection method thereof

Examples

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Embodiment 1

[0095] Example 1: Simultaneous detection device for immunoassay combined with white blood cell count

[0096] The synchronous detection device for immune detection combined with white blood cell counting provided in this embodiment includes a housing, a main control board, a detection card, a photoelectric detection unit (including an immune detection module and a total number of white blood cell detection module), a data processing unit, and a human-computer interaction unit, wherein The main control board is arranged in the casing, and the photoelectric detection unit, the data processing unit and the human-computer interaction unit are electrically connected to the main control board. Let me repeat.

[0097]The light source board 7 of the immune detection module in the photoelectric detection unit is an array arrangement of patch LEDs, the number of which is 6, the spacing is 4mm, and the central wavelength of the LED emitted light is 480nm; the central wavelength of the fi...

Embodiment 2

[0099] Example 2: Immunological detection combined with white blood cell count

[0100] This embodiment adopts the synchronous detection device of immunoassay combined with cell counting provided in Example 1 to perform immunoassay combined with white blood cell count, specifically including the following steps:

[0101] 1) Sample making

[0102] Use a sampling device such as a pipette to take 100 μL of blood sample, add it to the total sampling port 1c of the test card 1, or add it to the first sampling hole 216 and the second sampling hole 217 respectively, and a part of the blood sample (10 μL) will automatically flow into the In the test chamber at the total leukocyte count detection area 1d place, hemolysis and staining reactions are completed with the reagent (this embodiment is SYTO9 dye and quaternary ammonium salt) pre-packaged in the test chamber 22 of the total leukocyte count detection sheet 400; other blood samples will flow to the immunological Reaction area, wi...

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Abstract

The invention discloses a synchronous detection device combining immunodetection with leukocyte counting and a detection method thereof, and belongs to the technical field of analysis and detection. When the device provided by the invention is in use, immunological index detection and total leukocyte counting can be automatically and synchronously completed only by dropwise adding a detected bloodsample into the sample adding opening of a detection card, so the operation is simple, and the detection efficiency is high. The device provided by the invention is simple in structure and convenientto carry, and can be suitable for bedside detection, primary diagnosis and treatment and sick and wounded condition detection in a special environment. The detection method using the device is long in detection time window and is not interfered by impurities, so the detection accuracy is improved.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a synchronous detection device for immunological detection combined with white blood cell counting and a detection method thereof, which can simultaneously perform immune detection and total white blood cell counting. Background technique [0002] C-reactive protein (CRP) exists in a healthy body in trace amounts and participates in systemic inflammatory reactions. When acute trauma, inflammation and infection occur, the concentration of CRP in the blood will rise rapidly. It is a commonly used clinical It is a response index in the acute phase, and can be detected 6h-12h after the inflammation begins. Initially, the detection of CRP is mainly used in the diagnosis of active rheumatic fever. Due to the limited detection results and low sensitivity, it was initially used However, with the development of immunology and analytical biology in recent years, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64G01N33/577
CPCG01N15/1434G01N21/6486G01N33/577G01N2015/1006G01N2015/1486
Inventor 李悦琴
Owner 苏州中科苏净生物技术有限公司
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