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Separation and identification method of dermal papilla cells of cashmere goats

A technology of hair papilla cells and identification methods, which is applied in the field of identification and separation of cashmere hair papilla cells, can solve the problems of unsatisfactory identification results of DP cells, and achieve accurate identification results

Active Publication Date: 2020-11-10
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creating cell-like structures called papillons (or pearls) from animal tissue such as cow's milk or sheep skin by cutting them into small pieces with specific tools like scissors or knives. These tiny fragments are then separated out through various methods until they meet certain criteria set up on their way back home. By combining these markers genetic material onto this structure it becomes possible to make rapid and accurate identifications of different types of cells found within cassum lining fibers during production processes.

Problems solved by technology

This patents discusses how understanding the structure or activity of specific types of cells called dendritic cells plays crucial roles during embryonic processes such as hair foll formation and renewing. These cells help create structures like blood vessels and nerves within mature skin tissue when they develop into hairs. Additionally, these cells act as signal centres controlling various aspects of this process including activation of genes associated with them.

Method used

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  • Separation and identification method of dermal papilla cells of cashmere goats
  • Separation and identification method of dermal papilla cells of cashmere goats

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Experimental program
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Embodiment

[0037]1. Isolation of cashmere goat DP cells: cut the dorsal skin tissue of the cashmere goat scapula, and repeatedly wash the skin tissue with PBS solution containing 1% (w / v) double antibodies (penicillin, streptomycin) to remove surface stains . Afterwards, they were placed in DMEM / F12 medium containing 1% (w / v) penicillin and streptomycin, and transported on ice to an ultra-clean working environment. After entering the ultra-clean workbench, use ophthalmic forceps to remove residual blood stains and impurities on the skin tissue, and brush the tissue sample with DMEM / F12 medium containing 1% penicillin and streptomycin. Subsequently, the skin tissue was cut into long strips along the growth direction of the hair follicle with a scalpel, with a thickness of about 2-3 mm, and the tissue was washed again with DMEM / F12 medium containing 1% (w / v) penicillin and streptomycin.

[0038] The skin tissue was transferred to 2 mg / mL collagenase solution and digested in a constant tem...

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PUM

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Abstract

The invention belongs to the field of cell culture, and in particular relates to a separation and identification method of dermal papilla cells of cashmere goats. The specific technical scheme is thatSOX<2+> and/or FGF<7+> and/or APOD<+> and/or HHIP<+> are/is used as marker genes of dermal papilla cells. The invention provides a method capable of accurately separating and identifying dermal papilla cells of cashmere goats for the first time. In terms of separation, an enzyme digestion method and a mechanical separation method are combined to jointly obtain cells; and furthermore, a culture medium special for culturing the dermal papilla cells of the cashmere goats is provided, so that required cells are rapidly obtained. In terms of identification, several new marker genes are jointly used for the first time in the invention, so that the required cells can be rapidly and accurately identified.

Description

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Claims

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Application Information

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Owner NORTHWEST A & F UNIV
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