Method and device for eliminating out-of-focus diffusion effect in microscopic imaging scene

Abstract

The invention provides a method and device for eliminating the defocus diffusion effect in a microscopic imaging scene, belonging to the fields of image processing and artificial intelligence. The method includes: generating a decision matrix according to the image set to be fused; establishing a background matrix with the same size as the image to be fused, obtaining background pixels with low pixel values ​​in the image set to be fused according to the image set to be fused and the decision matrix, and It is recorded in the background matrix; a preliminary image fusion result graph is generated according to the decision matrix, and the result recorded in the background matrix is ​​overlaid into the preliminary image fusion result to obtain an image fusion result graph after eliminating the out-of-focus diffusion effect. The present invention can solve the problem of artifacts in the background caused by the out-of-focus diffusion effect of the foreground in the multi-focus image fusion task in the microscopic imaging scene without changing the original focus shooting interval and brightness. Thereby improving the imaging accuracy.

Description

technical field [0001] The present invention relates to the fields of image processing and artificial intelligence, in particular to a method and device for eliminating the out-of-focus diffusion effect in a microscopic imaging scene. Background technique [0002] Due to the limitation of the degree of freedom of the optical lens, it is difficult to focus all objects with large depth distances in one lens. Therefore, a multi-focus image fusion method based on image processing technology is usually used to fuse the respective clear areas in multiple images focusing on different targets in the same scene, and finally obtain a full-resolution image. This method is often used in image processing and analysis tasks in microscopic imaging scenarios, that is, due to the unevenness of the surface of the observed object, it is necessary to adjust the focal length of the microscope to take multiple images to obtain focused images of different targets, so multi-focus image fusion metho...

AI Technical Summary

Problems solved by technology

[0010] The embodiment of the present invention provides a method and device for eliminating the out-of-focus diffusion effect in a microscopic imaging scene, which can solve the multi-focus image fusion task in a microscopic imaging scene without changing the original focus shooting interval and brightness. The out-of-focus diffusion effect causes the problem of artifacts in the background in the fusion result, thereby improving imaging accuracy

Images