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Chilled meat freshness markers and their screening and prediction model fitting methods and uses

A prediction model, freshness technology, applied in character and pattern recognition, testing food, measuring devices, etc., can solve problems such as quality decline, endanger consumers' health, etc., to achieve reliable results, high accuracy and credibility, high resolution effects

Active Publication Date: 2022-07-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on metabolome profiling and freshness biomarkers in chilled fresh chicken storage
During the preservation process of chilled chicken, due to the action of microorganisms, the quality continues to decline, endangering the health of consumers

Method used

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  • Chilled meat freshness markers and their screening and prediction model fitting methods and uses
  • Chilled meat freshness markers and their screening and prediction model fitting methods and uses
  • Chilled meat freshness markers and their screening and prediction model fitting methods and uses

Examples

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Embodiment Construction

[0027] The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0028] The screening and prediction method of the marker in this embodiment includes the following steps:

[0029] 1. Experimental animals

[0030] The experimental animals were 70-day-old Haiyang yellow chicken hens raised by Jiangsu Jinghai Poultry Industry Group Co., Ltd., with a total of 32 hens under the same feeding conditions.

[0031] 2. Materials and methods

[0032] 2.1 Instruments and Reagents

[0033] Experimental instruments include: ultra-high performance liquid chromatograph (Agilent 1290), Q Exactive Orbitrap high-resolution mass spectrometer (Thermo Fisher), high-speed cryogenic centrifuge (Eppendorf), shaking vortex, 4°C refrigerator, pure water analyzer (TKA) ).

[0034] Experimental reagents include: acetonitrile (CMW, LCMS grade), ammonium acetate (CMW, LCMS grade), ammonia water (CMW, LCMS grade), methanol (CMW, LCM...

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Abstract

The invention discloses a metabolomics-based chilled meat freshness marker and a screening and prediction model fitting method and application thereof. The markers include indole-3-formaldehyde, uridylic acid, phenylthiouric acid, gluconic acid, tyramine, serine-phenylalanine. The prediction model is: Y=3.964+1.97E ‑7 X 1 ‑4.22E ‑7 X 2 ‑3.37E ‑7 X 3 +8.80E ‑8 X 4 +1.26E ‑8 X 5 ‑5.57E ‑7 X 6 , This method uses Agilent 1290 UHPLC tandem Q Exactive Orbitrap high-resolution mass spectrometry, which has higher resolution, can detect more substances more accurately, and can more comprehensively elucidate the metabolism of chilled chicken during storage. product, the results obtained are more reliable.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a chilled meat freshness marker and a screening and prediction model fitting method and application thereof. Background technique [0002] Chilled meat, also known as chilled meat, sour pork, chilled meat. It refers to the rapid cooling of the carcass after slaughter, so that the temperature of the carcass (the center of the hind leg meat is the measurement point) is reduced to 0-4°C within 24 hours, and it is always maintained at 0-4°C during subsequent processing, circulation and sales. Fresh meat in the range of 4°C. [0003] Chilled chicken refers to the quarantined live chicken, which is rapidly cooled after slaughtering to keep the core temperature of the chicken carcass at 0-4°C, and then divided, trimmed, packaged, and maintained during subsequent storage, transportation and sales. Fresh chicken in the 0-4°C range. The safety concerns associated with chilled chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N33/12G06V10/764G06F17/18
CPCG01N30/8693G01N33/12G06F17/18G06F18/24323
Inventor 张涛张闪闪陈兰谢恺舟张跟喜王金玉戴国俊巩用双
Owner YANGZHOU UNIV
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