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Method for observing growth morphological structure of flammulina velutipes hyphae

A morphological structure and technology of Flammulina velutipes, applied in the field of Flammulina velutipes, can solve the problems of chaotic mycelium and loss of fidelity

Pending Publication Date: 2020-10-23
江苏华绿生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the shape of mycelium can be seen by this method, because the mycelium has been disturbed during the film production process, the shape of the mycelium seen is different from the shape of the natural growth state of the mycelium, and the verisimilitude is lost. , there is a big disadvantage in understanding the real traits of mycelia

Method used

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  • Method for observing growth morphological structure of flammulina velutipes hyphae
  • Method for observing growth morphological structure of flammulina velutipes hyphae

Examples

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Embodiment 1

[0019] Such as Figure 1 to Figure 2 As shown, the present invention discloses a method for observing the growth morphology and structure of Flammulina velutipes mycelium, comprising the following steps, step 1, making of a slide culture chamber; step 2, sterilizing the slide culture chamber; step Three, preparation of agar block; Step four, inoculation; Step five, cultivation; Step six, microscopic examination; The making of described glass slide cultivation chamber is to spread a piece of circular filter paper sheet less than the bottom of the dish at the bottom of the plate culture dish. Put a support device on it, put a clean glass slide on the support device, then place two coverslips obliquely on both sides of the slide glass, and then cover the flat cover; culture the slides The chamber is sterilized by placing a plurality of prepared glass slide culture chambers in a sterilizing pot and sterilizing at 121°C for 30 minutes, and drying for later use; the supporting devic...

Embodiment 2

[0021] Such as Figure 1 to Figure 2 As shown, the present invention discloses a method for observing the growth morphology and structure of Flammulina velutipes mycelium, comprising the following steps, step 1, making of a slide culture chamber; step 2, sterilizing the slide culture chamber; step Three, preparation of agar block; Step four, inoculation; Step five, cultivation; Step six, microscopic examination; The making of described glass slide cultivation chamber is to spread a piece of circular filter paper sheet less than the bottom of the dish at the bottom of the plate culture dish. Put a support device on it, put a clean glass slide on the support device, then place two coverslips obliquely on both sides of the slide glass, then cover the flat cover, and culture the slide glass The chamber is sterilized by placing a plurality of prepared glass slide culture chambers in a sterilizing pot and sterilizing at 121°C for 30 minutes, and then drying for later use. The suppor...

Embodiment 3

[0023] Such as Figure 1 to Figure 2 As shown, the present invention discloses a method for observing the growth morphology and structure of Flammulina velutipes mycelium, comprising the following steps, step 1, making of a slide culture chamber; step 2, sterilizing the slide culture chamber; step Three, preparation of agar block; Step four, inoculation; Step five, cultivation; Step six, microscopic examination; The making of described glass slide cultivation chamber is to spread a piece of circular filter paper slightly smaller than the bottom of the dish at the bottom of the plate culture dish, Put a support device on it, put a clean glass slide on the support device, then place two coverslips obliquely on both sides of the slide glass, then cover the flat cover, To sterilize the culture chambers, a plurality of prepared glass slide culture chambers are placed in a sterilizer and sterilized at 121°C for 30 minutes, and dried for later use; the support device is a U-shaped gl...

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Abstract

The invention discloses a method for observing the growth morphological structure of flammulina velutipes hyphae. The method comprises the following steps: step 1, manufacturing a glass slide culturechamber; step 2, sterilizing the glass slide culture chamber; step 3, preparing an agar block; step 4, performing inoculation; step 5, performing culture: placing the inoculated glass slide culture chamber in an incubator for culture at 19+ / -0.5 DEG C, and after 3-5 days, taking out the chamber, performing observing by a microscope and recording a result; and step 6, performing microscopic examination. The flammulina velutipes hyphae cannot be disordered or damaged when the method observes the growth form of the flammulina velutipes hyphae, and compared with a traditional observation method, the form structure of the flammulina velutipes hyphae in the natural growth state can be observed more clearly.

Description

technical field [0001] The invention relates to the technical field of Flammulina velutipes, in particular to a method for observing the growth morphology and structure of Flammulina velutipes hyphae. Background technique [0002] Edible fungus hyphae are filaments composed of tubular cells, which are formed by germinating germ tubes after spores absorb water, and the tubular cells of the germ tubes continue to divide and elongate. Mycelium is a filamentous body made of numerous hyphae intertwined, generally in the shape of white fluff, with transverse septa in the hyphae, mostly multicellular, and each mycelial cell has a cell wall, cytoplasm and nucleus; the front end of the hyphae The mycelium group formed by continuous growth, branching and interweaving, commonly known as mycelium, is the vegetative body of edible fungi, which is the basis for the formation of fruiting bodies (fruiting). The quality of mycelium plays a decisive role in whether fruiting, yield, and quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/645
CPCC12Q1/04
Inventor 冯占朱琼李贺文余养朝
Owner 江苏华绿生物科技股份有限公司
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