SNP marker linked to branch angles of pedicels at bottom parts of cauliflower balls and method and application of SNP marker
A branching angle, cauliflower flower technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of long cycle and low efficiency
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Embodiment 1
[0059] A method for rapid identification of pedicel branching angle traits at the bottom of a cauliflower curd, the method comprising:
[0060] (1) Extraction of seedling genomic DNA
[0061] The seeds of the cauliflower germplasm material were sown in the plug trays, and the seedlings were raised by conventional methods, and the individual plants were numbered; the tender leaves of each individual plant were taken to extract genomic DNA according to the conventional CTAB (hexadecyltrimethylammonium bromide, hexadecyltrimethylammonium bromide) method. , -20 ℃ storage, spare.
[0062] (2) PCR amplification
[0063] PCR amplification was performed with the genomic DNA of each seedling as the template and ZAAS.SA01 as the primer pair. The PCR system adopts the KASP basic reaction system of the 96-well module: the total volume is 10 μL, including 1 μL DNA template (50 ng / μL), 5 μL KASPmastermix (2×), 0.14 μL primer pair mix, 4 μL ddH 2 O.
[0064] (3) Use LC480softwarev1.5.1 t...
Embodiment 2
[0066] A selected cauliflower variety 'Baiyu 80' (Taiwan Hefeng Seedling Co., Ltd. hybrid) and inbred line '4306-1' (Taiwan Qingnong Seedling Co., Ltd. hybrid 'Qingnong 90 days' inbred F 8 A molecular marker-assisted selection method for the branching angle of the pedicel at the bottom of the curd in the progeny of the hybrid progeny, including:
[0067] (1) The seeds of 'Baiyu 80' and '4306-1' were sown, nursed, and planted in plastic greenhouses; '4306-1' was used as the male parent, and 'Baiyu 80' was used as the female parent to carry out artificial budding hybridization at the flowering stage. After the seed pods are mature, the seeds are collected, and the seeds are planted in a plastic greenhouse the following year. When flowering, artificial pollination is performed and the seeds are harvested.
[0068] (2) The seeds harvested in step (1) were sown in plug trays, and seedlings were raised by conventional methods, and numbered by individual plants; genomic DNA was extra...
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