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Biomarker of active pulmonary tuberculosis, and application of biomarker

A technology for active pulmonary tuberculosis and biomarkers, which is applied in the field of biomarkers to achieve the effects of convenient collection, easy handling and economic burden reduction

Active Publication Date: 2020-10-16
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on has-miR-433-3p in peripheral blood mononuclear cells as a biomarker for active tuberculosis

Method used

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  • Biomarker of active pulmonary tuberculosis, and application of biomarker
  • Biomarker of active pulmonary tuberculosis, and application of biomarker
  • Biomarker of active pulmonary tuberculosis, and application of biomarker

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A biomarker of active pulmonary tuberculosis in this embodiment, see figure 1 , the biomarker is hsa-miR-433-3p.

[0041] Among them, the expression of hsa-miR-433-3p is significantly upregulated in APTB PBMCs, see for details figure 1 Shown in Figure A.

[0042] Among them, the hsa-miR-433-3p indicates early active pulmonary tuberculosis.

[0043] In this example, the up-regulated hsa-miR-433-3p is of great significance for the early diagnosis of APTB patients. Therefore, detecting the expression level of hsa-miR--433-3p in APTB patients by qPCR method can diagnose the disease, and then confirm that the hsa-miR-433-3p can be used to prepare active tuberculosis diagnostic reagents.

Embodiment 2

[0045] For the application of a biomarker for active pulmonary tuberculosis in this embodiment, see figure 1 and figure 2, the hsa-miR-433-3p in Example 1 was applied to the preparation of a diagnostic reagent for active pulmonary tuberculosis.

[0046] Compared with the existing detection techniques such as sputum smear microscopy and sputum culture, the active tuberculosis diagnostic reagent prepared by using the hsa-miR-433-3p has the following advantages: the collection of peripheral blood samples is convenient, and the peripheral blood samples are easy to obtain. Processing; the detection time is short, and the result can be obtained quickly, which is conducive to the rapid diagnosis of the disease; the detection cost is relatively low, which can reduce the economic burden of the patient.

Embodiment 3

[0048] For the application of a biomarker for active pulmonary tuberculosis in this embodiment, see Figure 2-4 , using hsa-miR-433-3p in Example 1 as a therapeutic target for active pulmonary tuberculosis. Using hsa-miR-433-3p as a therapeutic target for APTB is beneficial to the development of new drugs.

[0049] Among them, the potential downstream target genes of hsa-miR-433-3p are Rap1a and PDCD4.

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Abstract

The invention provides a biomarker for active pulmonary tuberculosis, and relates to the technical field of biomarkers and therapeutic targets. The biomarker is hsa-miR-433-3p, and the hsa-miR-433-3pcan be used for the preparation of a diagnostic reagent for active pulmonary tuberculosis, and the active pulmonary tuberculosis diagnostic reagent prepared by using the hsa-miR-433-3p has the following advantages: the collection of a peripheral blood specimen is convenient, and the peripheral blood specimen is easy to treat; the detection consumed time is short, a result can be obtained quickly,and the rapid diagnosis of diseases is facilitated; and the detection cost is relatively low, and the economic burden of patients can be reduced. In addition, the hsa-miR-433-3p can also be used as atherapeutic target for active pulmonary tuberculosis, and the development of new drugs is facilitated.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to a biomarker of active pulmonary tuberculosis and its application. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb). It is one of the top ten causes of death in the world and poses a serious threat to human health. According to the report of the 2019 Global Tuberculosis Research Report Conference, globally, an estimated 10 million people will suffer from tuberculosis in 2018. China is still a country with a high burden of tuberculosis, accounting for 9% of the global prevalence, second only to India. With the spread and development of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis, and the co-infection of AIDS and tuberculosis, the situation of tuberculosis prevention and control is becoming more and more severe. At present, clinical diagnosis of tuberculosis laboratories mainly use spu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6883C12Q1/04C12R1/32
CPCC12Q1/689C12Q1/6883C12Q2600/158C12Q2600/178C12Q2600/136Y02A50/30
Inventor 徐军发郑碧英罗红张俊爱
Owner GUANGDONG MEDICAL UNIV
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