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Stem cell culture perfusion device

A stem cell culture and perfusion technology, which is applied in the field of stem cell culture perfusion devices, can solve the problems of inconvenient nutrient solution dispersion, inconvenient cell sampling, uneven culture, etc., and achieves the effect of maintaining quality, improving effect, and maintaining preservation environment.

Pending Publication Date: 2020-10-16
湖北明德健康科技有限公司
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Problems solved by technology

[0003] However, the existing cell culture perfusion device still has the problem of uneven dispersion of the nutrient solution that is inconvenient to cultivate cells, and the problem that it is inconvenient to sample the cultured cells. Based on this, the present invention designs a stem cell culture perfusion device , to solve the above problem

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Embodiment Construction

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0023] see Figure 1-3 , the present invention provides a technical solution: a stem cell culture perfusion device, including a base 1, a spraying mechanism 2 and a sampling mechanism 3, the spraying mechanism 2 includes a nutrient solution tank 201, a motor 202, a rotating shaft 203, a rotating rod 204, and a connecting pump 205 , delivery pipe 206, nozzle 207, rotating disc 208 and gear 209, the top of the base 1 is provided with a perfusion box 4, and the nutrie...

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Abstract

The invention provides a stem cell culture perfusion device, and relates to the field of cell culture. The stem cell culture perfusion device comprises a base, a spraying mechanism and a sampling mechanism, wherein a perfusion box is arranged on the top of the base; a nutrient solution box is fixedly connected to the top of the perfusion box; a motor is fixedly connected to the top of the nutrientsolution box through a supporting plate; a rotating rod is mounted on the top of the perfusion box through a bearing; a connecting pump communicates with the right side of the nutrient solution box;a conveying pipe communicates with an output end of the connecting pump; the other end of the conveying pipe extends into the perfusion box; a rotating plate is fixedly connected to the lower portionof the conveying pipe; and a gear is fixedly connected to the bottom of the rotating rod and is engaged with the rotating plate. A nutrient solution stirred by a rotating shaft is discharged by a sprayer, the rotating rod is driven to rotate through a transmission wheel and a transmission belt, by transmission of the gear and the rotating plate, the sprayer rotates along the center of the rotatingplate, thus, the nutrient solution is uniformly sprayed to a culture solution on the bottom of the perfusion box, and the perfusion effect is improved.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a perfusion device for stem cell culture. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they can be divided into three types: Class; totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are not fully differentiated and immature cells with the potential to regenerate various tissues and organs and the human body. They are called "universal cells" in the medical field. The perfusion device for stem cell culture generally consists of several petri dishes or flasks. [0003] However, the existing cell culture perfusion device still has the probl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/34C12M1/32C12M1/02
CPCC12M23/48C12M27/02C12M29/06C12M33/00C12M41/24
Inventor 陈飞杨方园
Owner 湖北明德健康科技有限公司
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