Application of natural sulfated polysaccharide derived from marine organisms as drug for resisting coronavirus and induced diseases

A sulfated polysaccharide and coronavirus technology, applied in the field of biomedicine, can solve the problem of undiscovered anti-new coronavirus activity

Active Publication Date: 2020-10-16
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventor describes how certain naturally occurring compounds found in sea plants help prevent viruses like SARCs or MERS from infecting humans through their ability to bind with specific receptors called Spikes. These chemical substances were discovered earlier when it was identified they could make medicine more effective against other types of bacteria such as Escherichia coli. They may even stop these microorganism's growth altogether if needed without causing harmful side effectes.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing novel compounds capable of treating diseases like Coronovimses Disease due to their ability to stop them from causing harmfulness without affecting humans' immune system response.

Method used

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  • Application of natural sulfated polysaccharide derived from marine organisms as drug for resisting coronavirus and induced diseases
  • Application of natural sulfated polysaccharide derived from marine organisms as drug for resisting coronavirus and induced diseases
  • Application of natural sulfated polysaccharide derived from marine organisms as drug for resisting coronavirus and induced diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 The inhibitory effect of natural sulfated polysaccharides from marine organisms on SARS-CoV-2 spike protein

[0088] Based on the SARS-CoV-2-spike protein virus detection system, evaluate fucoidan sulfate (derived from brown algae), iota-carrageenan (derived from red algae), fucosylated chondroitin sulfate (derived from Sea cucumber), rhamnosan sulfate (derived from green algae) block the effect of SARS-CoV-2 infected cells.

[0089] The sulfated polysaccharide sample used in the present invention can be extracted by conventional reagents, methods and equipment in the technical field.

[0090] 1) Cell resuscitation culture: 293T / 17 human embryonic kidney cell line, calculated by weight percentage, was cultured by mixing 10% fetal bovine serum, 1% double antibody and the rest of DMEM medium, and cultured for two generations after resuscitation. .

[0091] 2) Viroid preparation: spread 293T / 17 human embryonic kidney cells in a 6-well cell culture plate, and wh...

Embodiment 2

[0100] Example 2 Inhibitory effect of natural sulfated polysaccharides from marine sources on SARS-CoV-2 main protease

[0101] The main protease of SARS-CoV-2 can hydrolyze the fluorescent polypeptide substrate to make the substrate fluorescent. The main protease activity was inhibited by natural sulfated polysaccharides from marine sources, and the fluorescence intensity was lower than that of the blank control. Among them, the main protease and the fluorescent polypeptide substrate can be obtained by the method reported in the literature W.Dai et al., Science 10.1126 / science.abb4489 (2020).

[0102] (1) Preparation of TE buffer solution

[0103] Add 100 μL of 0.5 M ethylenediaminetetraacetic acid disodium (EDTA-2Na) aqueous solution into 2.5 mL of 1 M Tris-HCl buffer solution, dilute to 50 mL, adjust the pH to 7.3, and filter through a 0.22 μm filter membrane Afterwards, store at -4°C.

[0104] (2) Screening of the inhibitory activity of sulfated polysaccharides from mar...

Embodiment 3

[0113] Example 3 Inhibition of Fucoidan Sulfate of Different Concentrations on SARS-CoV-2 Main Protease

[0114] (1) Inhibition experiment of fucoidan sulfate on main protease activity

[0115] Add 87 μL TE buffer solution to the 96-well cell culture plate, then add 1 μL main protease with a molar concentration of 19.7 μM, mix well, and add 2 μL H 2 O and nine seaweed-derived fucoidan sulfate aqueous solutions with a molar concentration ranging from 0.5nM to 45μM were added to each well in turn, shaken well, and after reacting at room temperature for 30min, 10μL of 20μM molar concentration was added to each well. Substrate, quickly use a microplate reader to detect the fluorescence intensity. The sulfated polysaccharide sample used in the present invention can be extracted by conventional reagents, methods and equipment in the technical field.

[0116] (2) Microplate reader detection conditions

[0117] Under the conditions of excitation wavelength 320nm, emission wavelengt...

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Abstract

The invention belongs to the field of biomedicine, and particularly relates to application of a natural sulfated polysaccharide derived from marine organisms as a drug for resisting coronavirus and induced diseases, and application of natural sulfated polysaccharide and pharmaceutically acceptable salts derived from marine organisms in the preparation of a drug combined with spike protein, preparation of an inhibitor of main protease (Mpro), or preparation of a papain-like protease (PLpro) inhibitor. The natural sulfated polysaccharide derived from marine organisms has an obvious effect of inhibiting the proliferation of coronaviruses, and can not only inhibit the spike protein (Spike) on the surface of the coronavirus from binding to host cells, but also inhibit the activity of the MPro and (PLpro), thereby effectively blocking the infection of host cells by coronavirus.

Description

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Claims

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Application Information

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Owner OCEAN UNIV OF CHINA
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