Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus longum lysinus, microbial agent, biocontrol agent, preparation method and application thereof

A technology of Bacillus lysinus and microbial agent, applied in the field of microorganisms, can solve the problems of high difficulty and high cost, and achieve the effects of reducing pollution, simple and convenient application, and no phytotoxicity to crops

Active Publication Date: 2021-11-30
HUNAN PLANT PROTECTION INST
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a long-form lysine bacillus, microbial agent, biocontrol agent and its preparation method and application, in order to solve the existing technical problems of difficulty and high cost in preventing and treating plant nematode diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus longum lysinus, microbial agent, biocontrol agent, preparation method and application thereof
  • Bacillus longum lysinus, microbial agent, biocontrol agent, preparation method and application thereof
  • Bacillus longum lysinus, microbial agent, biocontrol agent, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] S4. Preparation of fermented liquid: collecting the fermented liquid in the fermentation culture in step S3 to obtain the microbial bacterial agent of Bacillus longum lysinus;

[0050] In this embodiment, the temperature of the slant seed cultivation in step S1 is 25°C-30°C, and the cultivation time is 30h-40h; the temperature of the liquid seed cultivation in step S2 is 25°C-30°C, and the oscillation speed is 180r / min ~250r / min, the cultivation time is 20h~30h; the temperature of the fermentation culture in step S3 is 25℃~30℃, the oscillation speed is 180r / min~250r / min, the cultivation time is 65h~75h, the inoculation amount of seed culture solution 2% to 5%.

[0051] According to another aspect of the present invention, there is also provided a biocontrol agent for Bacillus longum. The above-mentioned microbial agent of Bacillus longum is centrifuged, and the supernatant is collected and filtered to obtain a long-form Biocontrol agent for Bacillus lysinus. Centrifug...

Embodiment 1

[0081] Cultivate overnight with Escherichia coli (JM109) preserved in the laboratory, collect the bacteria, spread on the NGM medium, use slender and soft feathers to spread Caenorhabditis elegans on the NGM medium with Escherichia coli, place Cultivate in an incubator at 20°C to 25°C for 3d to 5d. Before treatment, sterilize Caenorhabditis elegans with 1mg / mL streptomycin sulfate for 15min-20min, and wash with sterile deionized water for 3 times.

[0082] NGM medium: A. Configure the following reagents: 1M KPO 4 Buffer: 108.3g KH 2 PO 4 , 35.6g K 2 HPO 4 Add water to 1 L (autoclave), 1M MgSO 4 (autoclaved), 1M CaCl 2 (autoclaved), 5mg / ml cholesterol (prepared with absolute ethanol, filtered after preparation, and stored at 4°C); B. Weigh 3g NaCl, 2.5g peptone and 20g Agar into a conical flask, add 975mlH 2 O, autoclave, 121°C for 20min; C. When the medium is cooled to 55°C, add 25ml 1M KPO 4 Buffer, 1ml 1MCaCl 2 , 1ml 1M MgSO4, 1ml 5mg / ml cholesterol, mix well.

[0...

Embodiment 2

[0091] Add the biocontrol agent of Bacillus longum C1 into the 24-well plate, add 900 μl to each well, then take 100 eggs of Meloidogyne incognita and mix it evenly with the biocontrol agent of Bacillus longum C1, repeat three times , set beef extract peptone liquid medium as a control; place the 24-well plate in a 25°C incubator, observe and count under a stereomicroscope after 5 days, and calculate the hatching rate and relative inhibition rate of Meloidogyne incognita eggs. In the following formula, root-knot nematode incognita is referred to as nematode for short.

[0092] Egg hatching rate (%) = average number of hatched nematodes ÷ average number of eggs × 100

[0093] Relative inhibition rate (%) = (average number of hatched nematodes in the control group - average number of hatched nematodes in the treatment group) ÷ average number of hatched nematodes in the control group × 100

[0094] The hatching of M. incognita eggs by Lysinacillus longum C1 biocontrol agents is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a long-form lysine bacillus, a microbial agent, a biocontrol agent, and a preparation method and application thereof. It is identified as a long-form lysine bacillus (Lysinibacillus macrolides), the strain is named C1, and is preserved in General Microbiology Center of China Committee for Culture Collection of Microorganisms, the preservation number is: CGMCC No.18349, and the preservation date is July 29, 2019. The lysine bacillus of the present invention provides a new strain of lysine bacillus C1, which has good activity of preventing and controlling plant root-knot nematodes, and lays a foundation for the research and development of plant nematode biological control. Moreover, the bacterial strain has good characteristics of promoting crop growth, which provides a basis for the application of microbial fertilizers.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a lysine bacillus longum. In addition, the present invention also relates to a microbial agent comprising the above-mentioned Bacillus longum lysinus, a biocontrol agent, and a preparation method and application thereof. Background technique [0002] Plant-parasitic nematodes (Plant-parasitic nematodes) are a class of obligate plant-parasitic pathogenic nematodes, which commonly occur in the world and cause huge economic losses to the production of agricultural economic crops. The annual economic losses caused by plant-parasitic nematodes worldwide About $150 billion. Root-knot nematode is considered to be the most harmful plant pathogenic nematode. It can parasitize more than 3000 kinds of plants including vegetables, food crops, cash crops, fruit trees, ornamental crops and weeds. After the disease occurs, the yield is generally reduced by 10 % or so, seriously as high as 75%. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00A01P21/00C05F11/08C05G3/60C12R1/01
CPCA01N63/20C05F11/08C12N1/20C05G3/60C12N1/205C12R2001/01
Inventor 程菊娥
Owner HUNAN PLANT PROTECTION INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com