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Method for identifying abnormal energy metabolism pathway of depression based on stable isotope tracing metabonomics

A stable isotope and energy metabolism technology, applied in the field of identification of abnormal energy metabolism pathways in depression based on stable isotope tracer metabolomics, can solve the problem of being unable to distinguish which metabolic pathway the metabolites come from

Active Publication Date: 2020-09-29
SHANXI UNIV
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Problems solved by technology

The combination of stable isotope tracing technology and metabolomics technology can just make up for the defect that conventional metabolomics can only detect the sum of terminal metabolites, but cannot distinguish which metabolic pathway the metabolites come from

Method used

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  • Method for identifying abnormal energy metabolism pathway of depression based on stable isotope tracing metabonomics
  • Method for identifying abnormal energy metabolism pathway of depression based on stable isotope tracing metabonomics
  • Method for identifying abnormal energy metabolism pathway of depression based on stable isotope tracing metabonomics

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Embodiment 1

[0030] Example 1: Method for identifying abnormal pathways of energy metabolism in depression based on stable isotope tracing, labeling energy metabolism precursor substance glucose with stable isotope, using normal phase chromatography column and reverse phase chromatography column to comprehensively characterize energy metabolism compounds, according to The isotopic distribution characteristics of the downstream metabolites of glucose clarify the exact metabolic pathways of abnormal energy metabolism in depression.

[0031] details as follows:

[0032] (1) Put the labeled energy metabolism precursor material 13 C 6 -Glucose was introduced into depression-like rats and blank control rats, samples were collected after 6 hours of continuous tail vein bolus injection, and unlabeled samples were collected before the introduction of the isotope tracer for subsequent identification.

[0033] (2) Using high performance liquid chromatography-mass spectrometry (HPLC-MS) full scan mode for da...

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Abstract

The invention belongs to the technical field of stable isotope tracing metabonomics, and provides a method for identifying an abnormal energy metabolism pathway of depression based on stable isotope tracing. The method comprises the steps of carrying out stable isotope labeling on an energy metabolism precursor substance glucose, carrying out comprehensive characterization on an energy metabolismcompound by utilizing a normal-phase chromatographic column and a reversed-phase chromatographic column, and determining an exact metabolic pathway of energy metabolism abnormality of depression according to isotope distribution characteristics of glucose downstream metabolites. The method provided by the invention has important scientific value for knowing the pathogenesis of depression and researching and developing novel antidepressant drugs, and provides a new method for exploring the action mechanism of diseases, discovering drug targets and researching and developing new drugs.

Description

Technical field [0001] The invention belongs to the technical field of stable isotope tracer metabonomics, and specifically relates to a method for identifying abnormal energy metabolism pathways in depression based on stable isotope tracer metabonomics. Based on this technology's research on abnormal pathways of energy metabolism in depression, another way to understand the pathogenesis of depression and discover potential targets of diseases. Background technique [0002] The serious harm of depression to human life has attracted great attention, but due to the unclear and complicated pathogenesis, the existing marketed antidepressants developed based on the monoamine neurotransmitter theory are not ideal, and the drug control rate is only about 30% Although a variety of pathogenesis has been developed, such as neuroendocrine, neuroimmunity, neurotrophic, inflammation hypotheses, etc., there is no ideal antidepressant drug developed based on these pathogenesis hypotheses. It s...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8682
Inventor 令狐婷秦雪梅田俊生高耀张丽增
Owner SHANXI UNIV
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