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Method for rapidly identifying Orobanche Cumana resistance genes of sunflowers

A technology of resistance gene and sunflower, applied in the biological field, can solve the problems of long experimental period, difficult to observe the germination and parasitic process, and poor repeatability, and achieves the effect of strong practicability, strong promotion and application value.

Pending Publication Date: 2020-09-29
SYNGENTA BIO TECH CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It aims to solve the problems that the current sunflower resistance genes cannot be quickly identified, the germination and parasitic process of sunflower is difficult to observe, the experiment period is long, and the repeatability is poor.

Method used

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  • Method for rapidly identifying Orobanche Cumana resistance genes of sunflowers
  • Method for rapidly identifying Orobanche Cumana resistance genes of sunflowers
  • Method for rapidly identifying Orobanche Cumana resistance genes of sunflowers

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Embodiment Construction

[0071] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. All other implementations obtained by those skilled in the art based on the examples in this application belong to the protection scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0072] figure 1 The flow chart of a method for rapidly identifying the sunflower radang resistance gene provided by the present invention is shown. The method of operation of the specific steps of the method...

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Abstract

The invention relates to the field of plant tissue culture and transformation and gene function identification, in particular to identification of Orobanche Cumana resistance genes of sunflowers. Theinvention establishes a method for rapidly identifying Orobanche Cumana resistance genes of sunflowers under in-vitro conditions based on complexes obtained by transformation of roots of the sunflowers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for quickly identifying sunflower radang resistance genes under in vitro conditions based on the sunflower hair root complex system. Background technique [0002] Sunflower cumana (Orobanche cumana Wallr.), also known as poison root or rabbit crutches, is an annual herb belonging to the Orobanche cumana Wallr family of dicotyledonous plants. It is a non-photosynthetic root parasite unique to sunflower, which negatively affects the growth and development of sunflower (1) . Infected plants are dwarfed, thin, with reduced disk diameter or failure to form flower disks, and eventually the whole plant dies (2) . In Europe and Asia, more than 80% of production losses due to radang damage (3) . [0003] There are several ways to control Randang more or less, for example different crop management solutions can be used: soil insolation, biological control, and the use of herbicides...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 于鲲文琴刘清利陈希
Owner SYNGENTA BIO TECH CHINA
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