Application of circular RNA to regulation and control of pancreatic islet beta cell proliferation

A technology of beta cells and pancreatic islets, applied in the field of biomedicine, to achieve good application prospects and the effect of promoting proliferation

Active Publication Date: 2020-09-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical removal of part of pancreatic tissue is a common clinical treatment, and there is no specific drug for this type of disease

Method used

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  • Application of circular RNA to regulation and control of pancreatic islet beta cell proliferation
  • Application of circular RNA to regulation and control of pancreatic islet beta cell proliferation
  • Application of circular RNA to regulation and control of pancreatic islet beta cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this embodiment, mouse islet β-cell SJb is used as a model for illustration, so a mouse-derived circRNA, ie, circ_mmu_circ_0014773 (SEQ.ID.NO.2), was selected.

[0029] First, it was verified that circ_mmu_circ_0014773 has an endogenously expressed circular RNA sequence in SJb islet β cells. PCR amplification was carried out by designing specific primers for amplifying circular RNA, and the amplified product was sequenced by Sanger to obtain a circular sequence formed by the first link of the linear sequence. figure 1 The middle arrow shows the novel sequence formed by the combination of the last base and the first base of the linear sequence (looping interface sequence).

[0030] Overexpression of circ_mmu_circ_0014773 can effectively promote the proliferation of mouse islet β-cell SJb, and knocking out endogenous circ mmu_circ_0014773 can effectively inhibit the proliferation of mouse islet β-cell SJb. The overexpression and knockout vectors of circ mmu_circ_001477...

Embodiment 2

[0061] Overexpression of circ mmu_circ_0014773 can effectively promote the proliferation of SJb in mouse islet β cells, and knocking out mmu_circ_0014773 can effectively inhibit the proliferation of SJb in mouse islet β cells. The overexpression and knockout of circ_mmu_circ_0014773 were respectively constructed to stably express SJb in mouse islet β cells, and verified The effect of mmu_circ_0014773 on mouse islet β cell SJb proliferation can be further developed as a drug for treating diabetes and has broad application prospects.

[0062] The following experiments were used to verify:

[0063] 1) The effect of overexpression of mmu_circ_0014773 on the survival rate of islet β cells

[0064] Take the overexpressed circular mmu_circ_0014773 (circ_mmu_circ_0014773), overexpressed linear mmu_circ_0014773 (Linear_mmu_circ_0014773), control plasmid (pCDH) cells constructed above, and inoculate them in a 96-well plate, each well containing 100 μl medium, 10 4 The individual cell s...

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Abstract

The invention discloses application of circular RNA to regulation and control of pancreatic islet beta cell proliferation. The nucleotide sequence of the circular RNA is shown as SEQ.ID.NO.1 or SEQ.ID.NO.2. Tests prove that overexpression of the circular RNA can effectively promote proliferation of mouse pancreatic islet beta cells, and knockout of the circular RNA can effectively inhibit proliferation of the mouse pancreatic islet beta cells, so that the circular RNA can be used for preparing drugs for treating diabetes or pancreatic islet beta cytosis. A series of experiments prove that thecircular RNA can effectively promote proliferation of pancreatic islet beta cells, and essentially proves that the circular RNA has a wide application background in treatment of insufficiency in insulin secretion caused by reduction of the number of pancreatic islet beta cells. The invention can provide a good candidate drug for clinical treatment of diabetes caused by reduction of the number of islet beta cells, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of a circular RNA in regulating the proliferation of pancreatic islet β cells. Background technique [0002] Diabetes mellitus (DM) is a metabolic disease that seriously threatens human health. Its onset is due to absolute or relative insulin deficiency, which leads to increased blood sugar and leads to various tissues (especially eyes, kidneys, heart, blood vessels, and nerves). Chronic damage and dysfunction. [0003] Diabetes is mainly divided into type 1 and type 2 diabetes. The pathogenesis of type 1 diabetes is mainly the destruction of pancreatic beta cells caused by various reasons, resulting in absolute lack or significant reduction of insulin. Type 2 diabetes is the most common in my country, and its etiology includes insulin resistance, progressive insufficient secretion of insulin, or both. Whether it is type 1 or more common type 2 diabetes, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N15/113A61K31/713A61P5/50A61P3/08A61P3/10A61P3/00
CPCC12N5/0676C12N15/113A61K31/713A61P5/50A61P3/08A61P3/10A61P3/00C12N2500/40C12N2310/141
Inventor 贾世奇庄景燊陶卫华邓艳瑞
Owner JINAN UNIVERSITY
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