Green muscardine fungus preparation for killing spodoptera frugiperda

Abstract

The invention discloses a green muscardine fungus preparation for killing spodoptera frugiperda. The method comprises the following steps of: S1, separating from collected larvae of ailing spodopterafrugiperda to obtain a green muscardine fungus strain, culturing the green muscardine fungus strain on a Sabouraud Dextrose Agar medium, transferring conidia to a new culture medium for purification,and then carrying out single spore separation to obtain purified green muscardine fungus MRCNTS20190814; S2, mixing green muscardine fungi with vegetable oil, a surfactant and an antioxidant; and S3,collecting and detecting after the mixing is completed. The green muscardine fungus preparation has a scientific and reasonable structure, and is safe and convenient to use; the insecticidal efficiency of the green muscardine fungi can be obviously improved by the new proportion; the death rate of the oil agent can reach 50% or above after about 4 days, the death rate of the water agent still doesnot reach 50% after 8 days, and the problem that the insecticidal effect is reduced due to the fact that spores germinate too early due to the fact that a traditional spodoptera frugiperda fungal insecticide is generally an oil suspending agent and water is added for dilution in the release process is solved.

Description

technical field [0001] The invention relates to the technical field of microbial insecticides, in particular to a metarhizium fungus preparation for killing Spodoptera frugiperda. Background technique [0002] Insecticide refers to a medicament for killing pests, such as beetles, flies, grubs, nasal worms, collembola and nearly ten thousand other pests. The use of insecticides has gone through several stages: the first discovered They are natural insecticides and inorganic compounds, but they have a single effect, large dosage, and short duration; organic synthetic insecticides such as organochlorine, organophosphorus, and carbamate are characterized by high efficiency, high residue or low residue, Many of them have high acute toxicity to mammals; [0003] However, traditional fungal insecticides against Spodoptera frugiperda are generally oil suspensions, which are diluted with water during the release process, resulting in premature germination of spores and reducing the ...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a metarhizium anisopliae fungus preparation for killing Spodoptera frugiperda, which can effectively solve the problem that traditional Spodoptera frugiperda fungal insecticides are generally oil suspensions, which are diluted with water during the release process, resulting in The problem of premature germination of spores and reduced insecticidal effect

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