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Method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis

A technology of Bacillus subtilis and collagen oligopeptides, applied in the biological field, can solve the problems of uneven molecular weight distribution of collagen oligopeptides, high cost of collagen oligopeptides, unfavorable absorption and utilization, etc., and achieve easy absorption and utilization, high content of hydrophobic amino acids , to achieve the effect of making full use of

Inactive Publication Date: 2020-09-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after pretreatment by salt method, alkali method, acid method and other chemical methods, the molecular weight distribution of the extracted collagen oligopeptide is uneven and has a variety of by-products, which is not conducive to the absorption and utilization of the human body.
Research has found that some by-products also have toxicity such as carcinogenic and teratogenic
In addition, most of the enzymes for extracting collagen oligopeptides now use finished proteases, such as papain, trypsin, neutral protease, pepsin, alkaline protease, etc. The price of these proteases is relatively expensive. A large amount is used, which makes the whole production process down, and the cost of the obtained collagen oligopeptide product is relatively high, which is not conducive to production

Method used

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  • Method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis
  • Method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis
  • Method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis

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Experimental program
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Effect test

Embodiment 1

[0041] The preparation of embodiment 1 pigskin collagen oligopeptide

[0042] Including the following steps:

[0043] (1) Pick a single colony of Bacillus subtilis and inoculate it in the seed medium, cultivate it on a shaking table for 12 hours at a temperature of 30° C. and a rotating speed of 150 r / min, then inoculate it in the fermentation medium with an inoculum size of 6%, and Cultivate for 48 hours at a temperature of 30°C and a rotational speed of 150r / min; collect the fermentation broth of Bacillus subtilis, use casein as a substrate, and use the tyrosine assay method to measure the enzymatic activity of collagenase, and measure the activity of Bacillus subtilis fermentation In the solution, the enzyme activity of collagenase is 509U / mL;

[0044] (2) After simply cleaning the pigskin, remove the hair on the surface of the pigskin and scrape off the fat on the surface of the pigskin with a knife, then grind the pigskin with a meat grinder, and finally dry the ground p...

Embodiment 2

[0049] The preparation of embodiment 2 fish skin collagen oligopeptides

[0050] Including the following steps:

[0051] (1) The preparation method of Bacillus subtilis fermented liquid is the same as embodiment 1, and the activity size of collagenase is 509U / mL;

[0052] (2) After the fish skin is simply cleaned, remove the fat on the surface of the fish skin and a little residual fish scale, then grind the fish skin with a meat grinder, dry the minced fish skin in an oven at 50°C, and store it at -20°C for later use , to get fish skin powder;

[0053] (3) Take fish skin powder, add distilled water according to the solid-liquid ratio of dried fish skin powder and distilled water 1:6.8g / mL, after mixing, sterilize the mixed solution at 121°C for 15 minutes to obtain the fish skin mixed solution;

[0054] (4) The step (1) of adding 34.3% of the mixed liquid volume of fish skin to hydrolyze the fish skin with the Bacillus subtilis fermented liquid, the hydrolysis time is 5h, a...

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Abstract

The invention discloses a method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis. The method comprises the following steps: carrying out fermentation cultureon bacillus subtilis to obtain bacillus subtilis fermentation liquor, and hydrolyzing animal skin by using the bacillus subtilis fermentation liquor to obtain the collagen oligopeptide which is smallin molecular weight, high in purity and easy to absorb by a human body. In the method provided by the invention, the pretreatment of the animal skin only needs simple mechanical treatment for removingsurface impurities, and does not need chemical pretreatment for degreasing and removing impure protein by using chemical reagents such as salt, alkali, acid and the like, so that the problem of environmental pollution caused by the use of the chemical reagents in the process of preparing the collagen oligopeptide is avoided; and meanwhile, in the method provided by the invention, expensive commercial protease does not need to be added, so that the production cost is remarkably reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing collagen oligopeptides by using bacillus subtilis to hydrolyze animal skin. Background technique [0002] Under the action of chemical action such as acid, alkali or bacteria, enzyme, the molecular chain of collagen disintegrates and breaks to form a product between amino acid and protein, which is collagen oligopeptide, the collagen oligopeptide The molecular weight is generally distributed in the range of 100-10000Da. The shorter the collagen oligopeptide chain and the lower the molecular weight, the easier it is to be effectively absorbed by the human body. The molecular weight of the main collagen oligopeptide powder currently on the market is generally in the range of 3000Da-5000Da. The molecular weight of collagen oligopeptide mainly depends on the reaction method, time and temperature. Collagen oligopeptides can be widely used in food, medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P21/06C07K1/34C12R1/125
CPCC07K1/34C12P21/00C12P21/06
Inventor 毕桂灿谢君
Owner SOUTH CHINA AGRI UNIV
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