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Method for accelerating crossbreeding process of strong winter wheat in four generations in one year in embryo culture technology

A technology of hybrid breeding and strong winter character, which is applied in the field of crop breeding, can solve the problems of long breeding cycle, achieve the effects of simple operation, speed up the process of hybrid breeding of strong winter character wheat, and shorten the growth period

Pending Publication Date: 2020-09-08
SHIHEZI UNIVERSITY +1
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Problems solved by technology

This is also the reason why hybrid breeding of strong winter wheat uses field selection to breed new varieties, and the breeding cycle is relatively long. Generally, it takes at least 8-10 years at the fastest to breed new varieties.

Method used

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  • Method for accelerating crossbreeding process of strong winter wheat in four generations in one year in embryo culture technology
  • Method for accelerating crossbreeding process of strong winter wheat in four generations in one year in embryo culture technology

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Embodiment Construction

[0026] Take strong winter wheat as example below to describe the specific implementation of the technology of the present invention in detail:

[0027] 1. Preparation for wheat immature embryo culture. The commercially available 1 / 2MS medium was used as the substrate for the culture of immature embryo meristems, sterilized at high temperature, and divided into sterile petri dishes for future use. Select wheat ears with consistent flowering marks, peel off the wheat kernels 10-15 days after flowering in the middle of the ears, store them in a refrigerator at 4°C for 1-2 days, and then sterilize them on an ultra-clean workbench: soak them in 75% alcohol for 2 days -3min, then rinse with sterile water. with 0.1% HgCl 2 The solution is disinfected and soaked for 8-10 minutes, then rinsed with sterile water 2-3 times, and irradiated with ultraviolet light for 20-30 minutes.

[0028] 2. Picking and inoculation of young wheat embryos. The sterilized grains were placed in a steril...

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Abstract

The invention belongs to agricultural scientific research technology, and relates to the field of crop breeding science, in particular to a method suitable for accelerating the crossbreeding process of strong winter wheat in four generations in one year in an embryo culture technology. The method mainly comprises the following steps: step one, a wheat immature embryo culture technology; step two,seedling artificial vernalization technology; step three, a wheat plant reproductive growth regulation and control technology; and step four, a gene homozygosis accelerating technology for four generations of a crossbreeding generation system in one year. The method is easy and convenient to operate, controllable in procedure, economical and practical, completely aiming at the strong winter wheatby utilizing the embryo culture technology, the artificial vernalization technology, the reproductive growth regulation and control technology and the gene homozygosis accelerating technology for fourgenerations of a crossbreeding generation system in one year, and the growth period of the strong winter wheat is greatly shortened under the controllable indoor culture condition, the crossbreedingprocess of the strong winter wheat is accelerated, and the breeding of four generations of strong winter wheat in one year is realized.

Description

technical field [0001] The invention belongs to agricultural scientific research technology, and relates to the field of crop breeding. In particular, it is a method for accelerating the process of hybrid breeding of strong winter wheat, which is suitable for embryo culture technology and has 1 year and 4 generations. Background technique [0002] Wheat embryo culture technology is a commonly used technology in wheat breeding and research. This technology can speed up the breeding process of breeding and scientific research materials, and provide experimental materials for studying the growth and development, morphological structure, physiological and biochemical, genetic variation of winter wheat breeding materials and accelerating the breeding process. Therefore, winter wheat embryo culture technology is a basic experimental technology for wheat breeding and scientific research. [0003] Embryo culture technology of winter wheat, using embryos as regenerated explants for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 李诚付凯勇李春艳
Owner SHIHEZI UNIVERSITY
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